中药参姜止痢合剂主要成分含量检测标准研究

doi:10.16431/j.cnki.1671-7236.2023.08.040

Abstract

Abstract: 【Objective】 This study was aimed to establish a method for the identification and content determination of Shenjiang Zhili mixture,and to provide a basis for the quality standard of the preparation.【Method】 Thin-layer chromatography (TLC) was used to analyze the Sophora flavescens and Zingiberis Rhizoma for qualitative identification research,and high performance liquid chromatography (HPLC) method was utilized to determine the content of matrine and oxymatrine. 【Result】 TLC results showed that the spots of the test samples in the TLC of Sophora flavescens and Zingiberis Rhizoma were clear,and the corresponding reference substances showed consistent spots in the same position on the TLC plate,while the negative control samples did not show characteristic spots. HPLC results showed that matrine and oxymatrine had good chromatogram peaks. The linear range of matrine from 5 to 100 μg/mL (R²=0.9999) and oxymatrine from 5 to 100 μg/mL (R²=0.9999) were good. The relative standard deviation (RSD) value (n=6) of the instrument precision test was 1.56%,indicating that the precision of the instrument was good and could meet the requirements of the subsequent content determination.At 0,6,12,24,36 and 48 h after the preparation of the sample solution,the calculated RSD (n=6) was 2.25%,indicating that the total content of matrine and oxymatrine in the sample solution remained stable at room temperature for 48 h. Reproducibility test results RSD (n=6) was 2.47%,indicating good reproducibility of the sample preparation method. The average recovery of total matrine and oxymatrine was 99.17%,RSD (n=6) was 2.84%. 【Conclusion】 The results showed that the reproducibility of qualitative and quantitative analysis methods of Shenjiang Zhili mixture was good,the results were accurate and reliable,and it could be used to identify Sophora flavescens and Zingiberis Rhizoma in Shenjiang Zhili mixture and to determine matrine and oxymatrine content,so as to effectively control the quality of matrine.

Key words: Shenjiang Zhili mixture; quality standard; thin layer chromatography; high performance liquid chromatography

CLC Number:

S853.74

LIU Lian, GUO Zhiting, ZHANG Kang, ZHANG Kai, ZHANG Jingyan, WANG Lei, LI Jianxi. Study on Quality Standard for the Main Components of Traditional Chinese Medicine of Shenjiang Zhili Mixture[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3421-3429.

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Study on Quality Standard for the Main Components of Traditional Chinese Medicine of Shenjiang Zhili Mixture

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