单宁酸对猪卵母细胞体外成熟及胚胎发育能力的影响

doi:10.16431/j.cnki.1671-7236.2021.10.022

Abstract

Abstract: The aim of this study was to investigate the effect of tannin acid on porcine in vitro maturation (IVM) and their subsequent embryonic development. Thus, the porcine cumulus-oocyte complexes were supplemented with different concentration (0, 1, 10 and 100 μg/mL) of tannin acid during 42 h IVM, and then cumulus cells expansion, oocyte maturation rate, and the level of glutathione (GSH), reactive oxygen species (ROS) and growth differentiation factor 9 (GDF9) in oocytes were evaluated. Moreover, parthenogenetic activation (PA) and in vitro fertilization (IVF) were carried out, and cleavage rate, blastocyst formation rate and total cell number of blastocyst were evaluated. The results showed that when compared with the control group, 10 μg/mL tannin acid significantly increased the cumulus expansion index and 100 μg/mL tannin acid significantly decreased it (P<0.05). There was no significant difference in oocyte maturation rate between control, 1 and 10 μg/mL tannin acid groups (P>0.05), while 100 μg/mL tannin acid significantly decreased maturation rate (P<0.05). Moreover, the levels of GSH and GDF9 were dramatically enhanced (P<0.05), and the level of ROS was dramatically reduced by 1 and 10 μg/mL tannin acid supplementation (P<0.05). The results of development ability of parthenogenetic embryos and in vitro fertilization embryos showed that there was no significant difference in cleavage rate between tannic acid groups and control group (P>0.05). The blastocyst rate of parthenogenetic embryos and in vitro fertilization embryos in 10 μg/mL tannic acid group were significantly increased (P<0.05). The number of blastocyst cells in parthenogenetic embryos and in vitro fertilization embryos in 100 μg/mL tannic acid group was significantly lower than that in other groups (P<0.05). Taken together, 10 μg/mL tannin acid improved oocyte quality and the subsequent embryo developmental competence by increasing cumulus cells expansion and the GSH and GDF9 levels, and reducing the ROS level of the porcine oocyte.

Key words: tannin acid; pig; cumulus cell; oocyte; embryo development

CLC Number:

S828

SUN Jingtao, JIANG Chaoqian, LIU Jiahui, ZHANG Chi, WANG Chuanyue, BIAN Ya, JIANG Xiqing, ZHANG Qi, JIN Junxue. Effects of Tannin Acid on the in vitro Maturation and Embryonic Development of Porcine Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3708-3714.

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Effects of Tannin Acid on the in vitro Maturation and Embryonic Development of Porcine Oocytes

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