云南景洪帕利亚姆亚群达圭勒病毒的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2021.06.029

Abstract

Abstract: In order to understand the bovine infection with D’Aguilar virus (DAGV),BHK21 cells were used to isolate the virus from the blood samples of healthy cattle collected in Jinghong city,Yunnan province in 2019.The samples with cytopathy were identified by morphology,genomic banding and molecular biology.The sequences of S2,S3 and S7 genes were determined and compared.The results showed that five samples of CPE were found.The complete virus particles were observed by electron microscope,it was about 50 nm in diameter.The results of agarose gel electrophoresis showed that the genome of 5 newly isolated viruses was 10 segments,and the banding pattern of the virus genome was characterized by 3-3-4 electrophoresis.The banding pattern of the viruses were similar to that of the DAGV V106/YN/2014 strain isolated in Yunnan province in 2014.The nucleotide and amino acid sequences of S2,S3 and S7 genes of the five strains were 100%,the S3 and S7 genes had the highest homology with PALV isolated in China,and the S2 gene had the highest homology with DAGV isolated from Japan.Genetic analysis of S2,S3 and S7 genes showed that the similarity of the five newly isolated strains were 100%.The S7 and S3 genes were in the same branch with some known PALV strains isolated from China,indicating that these five strains were PALV.The S2 gene of five strains was located in the same branch as some DAGV strains isolated from Japan.It was further confirmed that these five strains were PALV DAGV.In this study,five strains of DAGV were successfully isolated,and the genetic evolution analysis of gene fragments were carried out to provide the basis for further epidemiological study of DAGV.

Key words: Palyamserogroup; D’Aguilar virus; isolation and identification

CLC Number:

S855.3

KOU Meiling, YANG Zhenxing, XIE Jiarui, LI Le, MIAO Haisheng. Isolation and Identification of D’Aguilar Virus from Palyamserogroup Virus in Jinghong,Yunnan Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2160-2168.

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Isolation and Identification of D’Aguilar Virus from Palyamserogroup Virus in Jinghong,Yunnan Province

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