猪成纤维细胞敲减卵泡抑素基因后的生物信息学分析

doi:10.16431/j.cnki.1671-7236.2021.03.003

Abstract

Abstract: In mammals,follistatin (FST) is not only involved in reproductive regulation,but also significantly associated with muscle growth and fat deposition.In order to explore the biological function of this gene in fibroblasts,the second generation sequencing technique was used to sequence the transcriptome of pig fibroblasts with FST gene knock-down and screen the differentially expressed genes. GO functional annotation and pathway enrichment analysis were carried out using on-line biological software.The results showed that 370 genes were significantly up-regulated and 287 genes were significantly down-regulated after FST gene knockdown.There were significant changes in HOXA6,PARP14,ZBP1,NDUFB9,RAB13,FAM83D,THBS1 and BTF3 genes associated with cell proliferation and apoptosis,as well as disease-related genes ZNF354C,RNF213,IFIT1,CCL5 and VPS13C.51 GO terms were clustered for these differential genes.Biological regulation,cellular process,metabolic process and single-organism process were screened under biological process.Organelle,cell part and cell were screened under cellular component.Binding terms were screened under molecular function.These terms were all including more than 200 differential genes.12 significantly enriched pathways were identified,including 3 pathways related to cell proliferation,such as p53 signal pathway,cell cycle and ribosome biogenesis in eukaryotes,4 pathways were related to disease,such as Alzheimer's disease,Salmonella infection,Huntington's disease and non-small cell lung cancer,2 pathways were related to muscle contraction,such as vascular smooth muscle contraction,cardiac muscle contraction.In addition,there were ubiquitin mediated proteolysis,regulation of actin cytoskeleton and gap junction pathways.The results indicated that after FST gene knockdown in fibroblasts,genes and biological pathways related to cell proliferation,differentiation and some diseases were significantly enriched,suggesting that FST gene played an important role in wound repair process of fibroblasts.

Key words: pig; fibroblast; follistatin gene; gene knock-down; second genetration sequencing

CLC Number:

S828.8

ZHANG Dongjie, SUN Yameng, WANG Liang, LI Zhongqiu, YANG Guowei, LIU Di. Bioinformatics Analysis of Porcine Fibroblasts After Follistatin Gene Knock-down[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 801-809.

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Bioinformatics Analysis of Porcine Fibroblasts After Follistatin Gene Knock-down

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