FNDC5在小鼠老龄化过程脂肪组织中的表达变化

doi:10.16431/j.cnki.1671-7236.2020.10.011

Abstract

Abstract: In order to investigate the expression changes of type Ⅲ fibronectin domain contains protein 5 (FNDC5) in adipose tissue of mice during aging process,healthy mice at different developmental stages were used as research objects.In this experiment,HE staining,immunohistochemical staining,Real-time PCR and Western blotting techniques were used to study the expression and localization of FNDC5 in adipose tissue during aging process.The results showed that FNDC5 was expressed to varying degrees in the fats of all developmental stages in the aging process of mice and showed significant differences.HE staining showed that with age,the size of fat cells gradually increased and then shrank.Immunohistochemical staining showed that the expression level of FNDC5 in adipose tissue of young mice was the highest,followed by that of sexual mature adipose tissue,and that of aged adipose tissue was lower than that of sexual maturation,and the expression level of FNDC5 was the lowest in mature adipose tissue of young mice.Subsequent Real-time PCR and Western blotting results were also consistent with immunohistochemical staining trends.The above results indicated that the FNDC5 gene might play a certain role in adipose tissue,which would provide new ideas for the study of fat metabolism and obesity-related diseases.

Key words: mice; adipose tissue; FNDC5; aging process; expression positioning

CLC Number:

Q786

LI Yilei, FAN Yunlin, LI Meiyi, MA Pei, JI Shusen, SUN Rui, LUO Xiaomao, WANG Haidong. Expression Changes of FNDC5 in Adipose Tissue During Aging Process in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3132-3139.

References 32

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Expression Changes of FNDC5 in Adipose Tissue During Aging Process in Mice

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