伴侣动物源肺炎克雷伯氏菌的分离鉴定及毒力和耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.05.033

Abstract

Abstract: In order to investigate the virulence and drug resistance of Klebsiella pneumoniae from Nanning companion animals,the fecal swabs of dogs and cats were collected,and the bacterial characteristics were analyzed by isolation and culture,morphological observation,drug sensitivity test and PCR amplification of 16S rRNA,khe gene,virulence genes and drug resistance genes.The results showed that four of the isolated strains could form a liquid colony on MacConkey medium,and they were short rod-shaped Gram-negative bacteria,suspected to be Klebsiella pneumoniae.The results of 16S rRNA sequencing showed that the homology between the isolated strain and Klebsiella pneumoniae was 99%,and Klebsiella pneumoniae specific gene (khe) was positive.Among them,GXKP-C14 and GXKP-D15 were sensitive to most commonly used antibiotics,GXKP-D1 showed high level of multi-drug resistance,followed by GXKP-D4 which showed ampicillin,piperacillin,cefuroxime,tetracycline,doxycycline,nitrofurantoin and sulfamethoxazole resistance,but sensitive to cefepime,meropenem,imipenem,amikacin,gentamicin,streptomycin and colistin.Some strains carried drug-resistant genes such as tet(A),QnrS,bla_SHV,sul2,mcr-1 and virulence gene WabG.The results of this study provided experimental basis for the detection,diagnosis and treatment of Klebsiella pneumoniae in dogs and cats.At the same time,the detection of colistin resistance gene mcr-1 would provide a reference for the control and rational use of polymyxin resistance.

Key words: Klebsiella pneumoniae; companion animals; multiple drug resistance; virulence

CLC Number:

S852.61

ZHANG Cong, XIAO Yichen, CHEN Huaijun, YUAN Jingzhi, WEI Deyuan, WANG Xiaoye. Isolation,Identification and Analysis of Virulence and Drug Resistance of Klebsiella pneumoniae from Companion Animals[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1583-1592.

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Isolation,Identification and Analysis of Virulence and Drug Resistance of Klebsiella pneumoniae from Companion Animals

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