甘蔗梢自然青贮过程中的营养成分及细菌多样性变化规律

doi:10.16431/j.cnki.1671-7236.2019.12.011

Abstract

Abstract: The objective of this study was to investigate the nutrient composition and bacterial diversity changes of natural fermented sugarcane tops silage under different silage period.Sugarcane tops silages were taken on 1st,15th,30th,45th,60th and 90th day.Nutrient contents,pH,lactic acid,ammoniacal nitrogen,volatile fatty acids VFA and microbial diversity were analyzed.The results showed that compared with sugarcane topssilageon the first day,DM content of sugarcane tops on 15th day was decreased significantly (P<0.05).Crude protein content was decreased with the extension of silage period (P<0.05).pH was decreased with the extension of silage period and was lower than 4.0 after 15-day fermentation (P<0.05).Lactic acid content was increased(P<0.05) and reached the highest value on 30th day,the highest value was 207.17 mmol/kg.Acetate content was increased with the extension of silage period (P<0.05) and no significant changes on propionate and butyric acid.On the first day,the dominant bacteria at order level of sugarcane tops silage were Lactobacillales,Rhizobiales,Bacillales,Micrococcales,Sphingomonadales order.Lactobacillales was the most dominant order after 15-day fermentation and Enterobacteriales was the second dominant order. Lactobacillus,Staphylococcus,Sphingomonas,Methylobacteriu,Rhizobium genus were the dominant genus in sugarcane tops silage on the first day.Lactobacillus was the most important dominant genus after 15-day fermentation.It can be concluded that sugarcane tops can get a good quality under natural silage conditions without any supplemented inoculums and Lactobacillus was an important bacteria genus in the natural fermented sugarcane tops silage.

Key words: sugarcane tops; natural fermented silage; nutrient; bacteria; diversity

CLC Number:

S816.5⁺3

LIANG Xin, TANG Qingfeng, GUO Yanxia, LI Mengwei, TANG Zhenhua, XIE Fang, PENG Kaiping, YANG Chengjian. Nutrient and Bacterial Diversity Changes of Natural Fermented Sugarcane Tops Silage[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3547-3554.

References

[1] 国家统计局.中国统计年鉴[M].北京:国家统计出版社,2018. The National Bureau of Statistics.China Statistical Yearbook[M].Beijing:National Statistical Press,2018.(in Chinese)
[2] 肖祎,吕达,陈政,等.甘蔗梢研究进展[J].中国糖料,2013,1:65-67. XIAO W,LYU D,CHENG Z,et al.Advances of sugarcane tops[J].Sugar Crops of China,2013,1:65-67.(in Chinese)
[3] 韦玉磊.充分利用甘蔗尾资源,大力发展南方肉牛产业[J].广西畜牧兽医,2012,28(6):361-362. WEI Y L.Development of beef cattle industry in sourthern of China by surgcane tops resources[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2012,28(6):361-362.(in Chinese)
[4] 蔡明,牟兰,王宗礼,等.甘蔗副产物的饲料化利用研究[J].家畜生态学报,2014,35(12):70-75. CAI M,MU L,WANG Z L,et al.Study on utilization of sugarcane by-products as forage[J].Acta Ecologiae Animalis Domastici,2014,35(12):70-75.(in Chinese)
[5] 邓卫东,席冬梅,刘勇,等.不同添加剂对甘蔗梢青贮营养价值的影响[J].中国畜牧杂志,2002,2:27-28. DENG W D,XI D M,LIU Y,et al.Effect of different additives on nutrient value of sugarcane tops silage[J].Chinese Journal of Animal Science,2002,2:27-28.(in Chinese)
[6] 李春霞.纤维酶制剂对甘蔗梢营养成分的影响[D].福州:福建农林大学,2010. LI C X.Effects of fiber enzymes on nutritions of sugarcane tip[D].Fuzhou:Fujian Agriculture and Forestry University,2010.(in Chinese)
[7] 唐振华,陈月丽,邹彩霞,等.热带假丝酵母、枯草芽孢杆菌与乳酸菌组合对甘蔗尾青贮品质和有氧稳定性的影响[J].中国畜牧兽医,2015,42(12):3217-3225. TAN Z H,CHENG Y L,ZOU C X,et al.Effect of Candida tropicalis,Bacillus subtilis and Lactobacillus combination on quality and aerobic stability of sugarcane tops silage[J].China Animal Husbandry & Veterinary Medicine,2015,42(12):3217-3225.(in Chinese)
[8] 朱欣.甘蔗梢饲料调制技术及其对湖羊生产性能研究[D].贵阳:贵州大学,2015. ZHU X.Modulation technology of sugarcane tips and study on productive performance of Hu sheep[D].Guiyang:Guizhou University,2015.(in Chinese)
[9] LEAL M R L,GALDOS M V,SCARPARE F V,et al.Sugarcane straw availability,quality,recovery and energy use:A literature review[J].Biomass and Bioenergy,2013,53:11-19.
[10] SHARIFI A,CHAJI M,MOHAMMADABADI T.The comparison of digestibility of treated sugarcane tops silage by bacteria or whole microorganisms of Holstein cow and buffalo rumen[A].Veterinary Research Forum[C].2016.
[11] COUTO J R,VILLELA S D,MOURTHE M H,et al.Sugarcane tops as a substitute for sugarcane in high-concentrate diets for beef bulls[J].Animal Production Science,2017,57(3):563-568.
[12] ORTIZ-RUBIO M,ØRSKOV E,MILNE J,et al.Effect of different sources of nitrogen on in situ degradability and feed intake of Zebu cattle fed sugarcane tops (Saccharum officinarum)[J].Animal Feed Science and Technology,2007,139(3-4):143-158.
[13] GENDLEY M,SINGH P,GARG A.Performance of crossbred cattle fed chopped green sugarcane tops and supplemented with wheat bran or lentil chuni concentrates[J].Asian-australasian Journal of Animal Sciences,2002,15(10):1422-1427.
[14] GENDLEY M,SINGH P,GARG A,et al.The studies on nutrient balances in crossbred cattle bulls fed chopped green sugarcane tops supplemented with some agro industrial by-products[J].Tropical Animal Health and Production,2009,41(6):943-949.
[15] YUANGKLANG C,WANAPAT M,WACHIRAPAKORN C.Effects of pelleted sugarcane tops on voluntary feed intake,digestibility and rumen fermentation in beef cattle[J].Asian-Australasian Journal of Animal Sciences,2005,18(1):22-26.
[16] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003. ZHANG L Y.Feed analysis and quality test technology[M].Beijing:China Agricultural University Press,2003.(in Chinese)
[17] 穆胜龙,杨冉冉,周波,等.植物乳杆菌和布氏乳杆菌对甘蔗尾青贮品质的影响[J].中国畜牧兽医,2018,45(5):1226-1233. MU S L,YANG R R,ZHOU B,et al.Effects of Lactobacillus plantarum and Lactobacillus buchneri on the quality of sugarcane tops silage[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1226-1233.(in Chinese)
[18] 藤井聪,岸原士郎,山口祯,等.甘蔗梢头部的成分[J].甘蔗糖业,1983,11:50-52. FUJII C,SHIRO K,YAMAGUCHI,et al.Composition of sugarcane tops[J].Sugarcane and Canesugar,1983,11:50-52.(in Chinese)
[19] 陈鑫珠,刘远,高承芳,等.不同添加剂对甘蔗梢青贮品质的影响[J].福建农业学报,2015,30(8):745-749. CHENG X Z,LIU Y,GAO C F,et al.Effect of additives on shelf-life and quality of sugarcane tips for forage[J].Fujian Journal of Agricultural Sciences,2015,30(8):745-749.(in Chinese)
[20] 余梅,刘建勇,赵刚,等.补饲精料对采食蔗梢青贮水牛消化代谢的影响[J].云南农业大学学报(自然科学),2013,28(4):470-475. YU M,LIU J Y,ZHAO G,et al.Effect of concentrate supplementation level on digestion and metabolism of buffalo fed sugarcane tops silage[J].Journal of Yunnan Agricultrual University (Natural Science),2013,28(4):470-475.(in Chinese)
[21] 唐振华,王波,邹彩霞,等.不同微生物添加剂组合对甘蔗尾青贮品质影响[J].饲料工业,2016,37(1):32-37. TANG Z H,WANG B,ZOU C X,et al.Efffect of microbial inoculants on quality of sugarcane tops silage[J].Feed Industry,2016,37(1):32-37.(in Chinese)
[22] 辛明,黄振勇,杨再位,等.甘蔗尾梢颗粒饲料对育肥牛、羊生长性能的影响[J].饲料工业,2017,38(11):46-50. XIN M,HUANG Z Y,YANG Z W,et al.Effects of sugarcane caudate loe pellet feed on the growth performance of fattening cattles and sheeps[J].Feed Industry,2017,38(11):46-50.(in Chinese)

Nutrient and Bacterial Diversity Changes of Natural Fermented Sugarcane Tops Silage

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GAO Shihua, WANG Fang, YIN Yexin, TAN Bi'e, YIN Yulong, CHEN Jiashun. Nutritional Value and Anti-nutrient Factor of Fermented Rapeseed Meal and Its Application in Animal Production [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2333-2341.
[2]	YANG Qinglin, WU Hao, LIU Jiayan, FU Yuxin, ZHOU Shuaishuai, LU Han, DING Manyi, LI Yao. Study of Quercetin Feed Additives on Prolonging Storage Time of Eggs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2342-2353.
[3]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[4]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[5]	LI Zhongbo, HOU Qianghong, DUAN Deyong. Haplotypes Diversity,Genetic Differentiation and Phylogenetic Analysis of Aedes albopictus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1452-1460.
[6]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[7]	GUO Bingyu, LI Li, LI Chen, KONG Xianghui. Role and Molecular Mechanism of Mpeg1 in Pathogen Clearance [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1584-1592.
[8]	WANG Wu, WANG Bi, BAI Yu, CHEN Qirong. Research Progress on Antibacterial Activity of Modified Lysozyme [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 942-951.
[9]	CHEN Shaojun, LI Gang, NAI Zida, LIU Di, JIANG Xinpeng. Research and Application of Gene-edited Probiotics in Animal Intestinal Health [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1016-1024.
[10]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[11]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[12]	YIN Yangyan, PAN Yan, LI Xiaoning, LI Jun, LIAO Yuying, LI Changting, WEI Tianchao, PENG Hao. Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 666-673.
[13]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[14]	WANG Jiahao, WANG Jing, YUAN Peng, LI Zhiwei, GENG Juan, WANG Hui, LI Fengming, ZANG Changjiang. Diversity Analysis of Fecal Flora in Young Suckling Camels and Adult Female Camels [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 97-105.
[15]	LIN Xiajing, ZHENG Chuntian, XI Pengbin, CHENG Zhonggang, DING Fayuan, RUAN Dong, WANG Yibing, JIANG Shouqun. Dietary Threonine Requirement of Yellow-feathered Broilers Aged From 22 to 42 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 134-142.