犬冠状病毒诊断技术研究进展

doi:10.16431/j.cnki.1671-7236.2019.12.007

Abstract

Abstract: Canine coronavirus (CCoV) is a common pathogen that causes gastrointestinal diseases in dogs,especially when co-infection with other intestinal pathogens,the mortality rate increases significantly,seriously affecting the healthy development of the canine industry.Fast,sensitive and specific diagnostic techniques play a vital role in the prevention and control of the disease.In addition to clinical diagnosis,the common CCoV laboratory diagnostic techniques mainly include virus isolation culture,electro-mirror observation and serological diagnostic techniques,however,these technologies often take a long time and a large workload,molecular diagnostic techniques mainly include ordinary RT-PCR,Real-time quantitative RT-PCR,nano PCR and other detection methods,these diagnostic techniques are greatly improved in accuracy,sensitivity,specificity and diagnostic efficiency.In recent years,with the rapid development of molecular immunodiagnostic technology and new materials development technology,new detection methods have emerged,nano antibody gold,nucleic acid probe,chip technology,and nano-biosensors and other technologies strive to be more sensitive,convenient,efficient and have the possibility of preparation kits,so as to be diagnostic concise and universal.This paper will provide a comprehensive review of the diagnostic techniques of CCoV,in order to provide reference for the development of CCoV diagnostic reagents.

Key words: coronavirus(CCoV); epidemiology; clinical diagnosis; laboratory diagnosis

CLC Number:

S858.292

HAO Yunfeng, YOU Xinyue, QIN Tong, CUI Shangjin. Research Progress on Diagnostic Techniques of Canine Coronavirus[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3514-3519.

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Research Progress on Diagnostic Techniques of Canine Coronavirus

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