狐狸流产奇异变形杆菌的分离鉴定及特征分析

doi:10.16431/j.cnki.1671-7236.2019.11.027

Abstract

Abstract: In order to clarify the main causes of fox abortion,identify the main pathogens and propose effective prevention and control measures,the spleen and ovaries of several aborted foxes in Hebei province were collected and analyzed by bacterial isolation,morphological observation,drug sensitivity test,16S rRNA gene sequencing,sequence analysis and homology alignment to isolate and identify the pathogens causing fox abortion and analyze its drug resistance to screen effective therapeutic drugs.At the same time,the pathogenicity of pathogens was tested.The results showed that a single Gram-negative bacterium was isolated from the spleen and ovaries of the aborted fox lesion,named as FOX-abortion-Hebei.The results of drug susceptibility test showed that the isolated strain was sensitive to 11 drugs such as cefoperazone,but was intermediate to 5 antibiotics such as tobramycin.The results of clinical animal tests showed that the strain had strong pathogenicity and could cause animal death in both mice and foxes.16S rRNA sequencing and homology analysis showed that the isolated Gram-negative bacteria were Proteus mirabilis (PM),which had a 93.6% to 99.9% homology with published sequences.The phylogenetic relationship between the bacteria and the published strain from Egypt (AUMC B-199) was the closest.The above research results showed that the main pathogen causing fox abortion was Proteus mirabilis,this bacterium was also isolated for the first time in fox abortion,which could cause serious reproductive disorders,it was necessary to do a good job of prevention and control in fox breeding.

Key words: fox abortion; Proteus mirabilis; pathogenicity; drug resistance

CLC Number:

S858.92

ZHANG Ping, CHENG Yuening, ZHANG Haiwei, QIAO Lianjiang, ZHOU Yucheng, XU Li, HOU Qingfeng, YANG Yanling. Isolation,Identification and Characteristics Analysis of a Proteus mirabilis from the Aborted Foxes[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3370-3377.

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Isolation,Identification and Characteristics Analysis of a Proteus mirabilis from the Aborted Foxes

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