猪胸膜肺炎放线杆菌血清8型菌株的分离鉴定及耐药性研究

doi:10.16431/j.cnki.1671-7236.2019.10.035

Abstract

Abstract: In order to ascertain the pathogen causing the death of fatten pigs in a large-scale pig farm in Taizhou,Jiangsu in May 2018,the lung tissues of the dead pigs were sterilized by the pathogen isolation,culture,stain observation,biochemical test and PCR amplification,and then the pathogenicity and drug resistance of the isolates were tested.The results showed that the isolated strain could only grow in the medium containing NAD.The bacteria could be found red through the Gram staining and microscope.The isolates were Gram-negative bacteria.According to the results of biochemical tests and PCR,the isolates were identified as Actinobacillus pleuropneumoniae.Serotype PCR showed that the isolates were Actinobacillus pleuropneumoniae serotype 8.The results of virulence test showed that BALB/c mice died when the concentration of the isolated strain reached 3×10⁸ CFU/mL,and the mortality rate of BALB/c mice was 100% when the concentration was 2×10⁹ CFU/mL.The results of susceptibility test showed that the strain was highly sensitive to 14 antimicrobial agents,such as cefotaxime,cefotaxime,aztreonam,cefepime,ceftriaxone and ofloxacin,and was moderately sensitive to ampicillin and resistant to streptomycin.The above results showed that there was infection of Actinobacillus pleuropneumoniae serotype 8 in the pig farm.

Key words: Actinbacillus pleuropneumoniae serotype 8 strain; isolation and identification; drug sensitive test

CLC Number:

S852.61

WANG Dandan, CHEN Guoqiang, ZHANG Changyin, YU Zhengyu, ZHU Haodan, ZHOU Junming, LYU Lixin, HE Kongwang, LI Bin, NI Yanxiu. Isolation,Identification and Drug Sensitive Tests of Actinbacillus pleuropneumoniae Serotype 8[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3124-3130.

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Isolation,Identification and Drug Sensitive Tests of Actinbacillus pleuropneumoniae Serotype 8

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