牦牛MDHⅠ基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2019.09.020

Abstract

Abstract: The aim of this study was to investigate the genetic polymorphism of MDHⅠ gene and its associations analysis with growth traits,in order to provide a reference for the breeding of qualify traits in yak.Five groups (178 total) of yak were selected,DNA were extracted from the ear samples,and DNA pools were constructed.The specific primers of all exons of MDHⅠ gene were designed for amplification and sequencing.SNPs were screened using Mega 5.2,the genotypes were identified by direct sequencing.χ² independence detection,gene homozygosity (Ho),heterozygosity (He),effective allele frequency (Ne) and polymorphism information the content (PIC) analysis were analyzed by PopGene 32,the association between the polymorphisms of MDHⅠ gene and growth traits in yak was analyzed using SPSS 24.0.The results showed that only one mutation site in exon 8 (G23093C,synonymous mutation) was found in Shenzha,Leiwuqi,Sibu,Pali and Maiwa yaks,which was synonymous mutations.There was three genotypes of GC,CC and GG,among the five yak populations,GC genotype was the dominant genotype (GG was the dominant genotype in Leiwuqi yak),and G was the dominant allele (except for Sibu yak),the gene frequency of G and C were equal in Sibu yak.χ² adaptability test showed that Shenzha,Leiwuqi,Sibu,Pali and Maiwa yaks were in accordance with Hardy-Weinberg equilibrium (P>0.05).MDHⅠ gene in Shenzha,Pali and Sibu yaks showed highly polymorphic (PIC>0.5),while Maiwa and Leiwuqi yaks showed moderately polymorphic (0.25 < PIC < 0.5),and the highest homozygosity was Shenzha and Leiwuqi yaks.The correlation analysis results showed that different genotypes of MDHⅠgene had significant effects on body weight in Leiwuqi,Pali and Maiwa yaks (P<0.05),and the interspecies analysis results showed that different genotypes of MDHⅠ gene had significant effects on body weight in yak (P<0.05).It indicated that MDHⅠ gene could be used as a molecular marker for artificial breeding.

Key words: yak; MDHⅠgene; polymorphism; growth traits; association analysis

CLC Number:

S823.8

CHEN Lulu, WANG Hui, CHAI Zhixin, ZHONG Jincheng, WANG Jikun, CHEN Zhihua. Polymorphisms of MDHⅠ Gene and Its Associations Analysis with Growth Traits in Yak[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2658-2664.

References

[1] 陈露露,王会,柴志欣,等.牦牛MDHⅠ基因的克隆及组织表达分析[J].生物技术通报,2019,35(2):129-136. CHEN L L,WANG H,CHAI Z X,et al.Cloning and tissue expression analysis of MDHⅠgene in yak[J].Biotechnology Bulletin,2019,35(2):129-136.(in Chinese)
[2] 钟金城.牦牛遗传与育种[M].成都:四川科学出版社,1996. ZHONG J C.Yak Genetics and Breeding[M].Chengdu:Sichuan Science Press,1996.(in Chinese)
[3] DING Y,MA Q.Characterization of a cytosolic malate dehydrogenase cDNA which encodes an isozyme toward oxaloacetate reduction in wheat[J].Bioehimie,2004,86(8):509-518.
[4] TOMITA T,FUSHINOBU S,KUZUYAMA T,et al.Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase mutant[J].Biochemical and Biophysical Research Communications,2006,347(2):502-508.
[5] GUAN H Y,TANG Z Q,LI H.Correlation analysis between single-nucleotide polymorphism of malate dehydrogenase gene 5'-flanking region and growth and body composition traits in chicken[J].Acta Genetics Sinica,2006,33(6):501-506.
[6] 刘大林.Apn、MDH和IGF-Ⅰ基因对京海黄鸡重要经济性状的遗传效应研究[D].扬州:扬州大学,2009. LIU D L.Research on genetic effect of Apn gene, MDH gene and IGF-Ⅰ gene on economical traits in Jinghai Yellow chicken[D].Yangzhou:Yangzhou University,2009.(in Chinese)
[7] 祝仁铸,尹逊河,王元虎,等.猪肌肉组织MDH和LPL基因表达与肌内脂肪含量和脂肪酸组成关系的研究[J].畜牧兽医学报,2013,44(8):1182-1188. ZHU R Z,YIN X H,WANG Y H,et al.The gene expression of MDH and LPL in muscle and their association with content of intramuscular fat and composition of fatty acids in pigs[J].Acta Veterinaria et Zootechnica Sinica,2013,44(8):1182-1188.(in Chinese)
[8] BAI C,WANG D,LI C,et al.Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank[J].European Journal of Histochemistry,2011,55(1):e4.
[9] ZHANG K,FENG G D,ZHAG B Y,et al.Application of epigenetic markers in molecular breeding of the swine[J].Yi Chuan,2016,38(7):634-643.
[10] WANG G,ZHAO X,ZHONG J,et al.Cloning and polymorphisms of yak lactate dehydrogenase B gene[J].International Journal of Molecular Sciences,2013,14(6):1994-2003.
[11] DING X Z,LIANG C N,GUO X,et al.A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers[J].Molecular Biology Reports,2012,39(1):669-673.
[12] MA Z J,CHEN S M,SUN Y G,et al.Y-STR INRA189 polymorphisms in chinese yak breeds[J].Genetics and Molecular Research,2015,14(4):18859-18862.
[13] CHU M,WU X Y,GUO X,et al.Association between single-nucleotide polymorphisms of fatty acid synthase gene and meat quality traits in datong yak (Bos grunniens)[J].Genetics and Molecular Research,2015,14(1):2617-2625.
[14] IMRAN M,TANG K,LIU J Y.Comparative genome-wide analysis of the malate dehydrogenase gene families in cotton[J].PLoS One,2016,11(11):e0166341.
[15] KIM E Y,HAN B S,KIM W K,et al.Acceleration of adipogenic differentiation via acetylation of malate dehydrogenase 2[J].Biochemical and Biophysical Research Communications,2013,441(1):77-82.
[16] VENKAT S,GREGORY C,STURGES J,et al.Studying the lysine acetylation of malate dehydrogenase[J].Journal of Molecular Biology,2017,429(9):1396-1405.
[17] HAN X,TONG Y,TIAN M,et al.Characterization of the immunogenicity and pathogenicity of malate dehydrogenase in Brucella abortus[J].World Journal of Microbiology and Biotechnology,2014,30(7):2063-2070.
[18] LIMA QUEIROZ A,ZHANG B,COMSTOCK D E,et al.miR-126-5p targets malate dehydrogenase 1 in non-small cell lung carcinomas[J].Biochemical and Biophysical Research Communications,2018,499(2):314-320.
[19] CASCON A,COMINO-MENDEZ I,CURRAS-FREIXES M,et al.Whole-exome sequencing identifies MDH2 as a new familial paraganglioma gene[J].Journal of the Nationnal Cancer Institute,2015,107(5):djv053.
[20] AIT-El-MKADEM S,DAYEM-QUERE M,GUSIC M,et al.Mutations in MDH2,encoding a Krebs cycle enzyme,cause early-onset severe encephalopathy[J].American Journal of Human Genetics,2017,100(1):151-159.
[21] 朱志飞.MDH、LDHC基因多态性与肉鸽生长及繁殖性状的相关性分析[D].扬州:扬州大学,2011. ZHU Z F.Correlation analysis between genetic polymorphisma of MDH and LDHC gene and growth and breeding traits of meat pigeons[D].Yangzhou:Yangzhou University,2011.(in Chinese)
[22] 梁伟.癌症相关的同义突变对基因翻译过程的影响[D].南京:东南大学,2012. LIANG W.The effect of cancer-associated synonymous mutations on gene translation processes[D].Nanjing:Southeast University,2012.(in Chinese)
[23] 易恒洁,李辉,赵忠海,等.高坡猪肌肉生长抑制素基因多态性及其与肉质性状的相关性分析[J].中国畜牧兽医,2017,44(4):1102-1107. YI H J,LI H,ZHAO Z H,et al.The polymorphisms of MSTN gene and its relationship with meat quanlity traits in Gaopo pig[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1102-1107.(in Chinese)

Polymorphisms of MDHⅠ Gene and Its Associations Analysis with Growth Traits in Yak

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[2]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[3]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[4]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[5]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[6]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[7]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[8]	WANG Yunlu, MENG Zhaoyi, YAO Yilong, GUO Min, NIU Jiaqaing, SUOLANG Sizhu, XU Yefen. Screening and Targeting Verification of lncRNA of "Sponge Adsorption" bta-miR-146a in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 859-869.
[9]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[10]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[11]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[12]	WEI Jia, BAI Qin, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHAO Hongwen, TAN Wu, LI Huade, XIE Rongqing, SHA Quan, JIANG Mingfeng, ZHANG Xiangfei. Effects of Different Weaning Strategies on Growth, Serum Biochemical Indexes and Antioxidant Capacity of Yak Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3400-3410.
[13]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[14]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[15]	BAO Qi, BAO Pengjia, MA Xiaoming, WU Xiaoyun, MENG Guangyao, CHU Min, CAO Hongmei, LANG Jianying, AN Yufeng, LIANG Chunnian, YAN Ping. Genetic Structure,Selection Signal Analysis and ROH Detection of Sunan Yak Population [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2992-3005.