SH2B1基因及miR-276-3p对猪背部脂肪沉积的影响

doi:10.16431/j.cnki.1671-7236.2019.07.019

Abstract

Abstract:

This study was aimed to investigate the expression of SH2B1 gene in the different tissues and backfat at various growth stages of pigs,and predict the effect of miR-276-3p which regulated SH2B1 gene on the expression of backfat in pigs.Real-time quantitative PCR was used to detect the relative expression of SH2B1 gene of six tissues in pigs,and in backfat of pigs at 30,60,90,120 and 180 d.The target regulation miRNA of SH2B1 gene was predicted and the regulation of SH2B1 gene by miR-276-3p was detected by Real-time quantitative PCR.The results showed that SH2B1 gene was expressed in all six tissues,with the highest expression in fat and the lowest expression in muscle.The expression of SH2B1 gene was expressed at various stages of growth and development in pigs.The expression level was low at the early stage (30 and 60 d) and high at the middle and late stages (90,120 and 180 d).The expression of SH2B1 gene was significantly higher at the middle and late stages than that at the early stage (P<0.05).The relative expression of miRNA-276-3p and SH2B1 gene in backfat tissue of pigs that in high or low backfat thickness group (HBFT or LBFT) was detected by Real-time quantitative PCR.The results showed that miRNA-276-3p and SH2B1 gene were differentially expressed in backfat tissue of pigs.The expression of miRNA-276-3p in high backfat thickness group was significantly lower than that in low backfat thickness group (P<0.05),while the expression of SH2B1 gene in high backfat thickness group was significantly higher than that in low backfat thickness group (P<0.05).miRNA-276-3p negatively regulated SH2B1 gene and affected the fat deposition on the back of pigs.This results provided a reference for further studying the molecular mechanism of fat deposition and backfat thickness difference in pigs.

Key words: pig; SH2B1 gene; miRNA-276-3p; backfat thickness

CLC Number:

S828

LIU Xin, GONG Jianfei, LI Hanmei, YAN Hua, ZHANG Longchao, WANG Ligang, ZHAO Fuping, GAO Hongmei, HOU Xinhua, WANG Lixian. Effects of SH2B1 Gene and miR-276-3p on Backfat Deposition in Pigs[J]. , 2019, 46(7): 2038-2044.

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Effects of SH2B1 Gene and miR-276-3p on Backfat Deposition in Pigs

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