3株猪源大肠埃希氏菌耐药基因的初步定位

doi:10.16431/j.cnki.1671-7236.2019.04.034

Abstract

Abstract:

This trial was aimed to preliminarily locate clinically isolated Escherichia coli (E.coli) resistant genes in pigs.Using conventional bacterial isolation and culture,16S rRNA PCR amplification and sequence analysis,the pathogens were isolated and identified from the uterus pus sent from three large-scale pig farms in Jiangxi province,the drug resistance genes of clinical isolates were preliminarily located by plasmid extraction,transformation of E.coli DH5α competent cells and drug susceptibility test.The results showed that 3 strains of E.coli were isolated and identified,among which JX-22 was only sensitive to ofloxacin and spectinomycin,and JX-26 was only sensitive to four drugs,such as streptomycin and ofloxacin,JX-28 was only sensitive to three drugs such as ofloxacin,all were multi-drug resistant bacteria.Three strains of E.coli could be purified to obtain plasmids of different molecular masses.The results of drug sensitivity test of isolates and plasmid transformants and E.coli DH5α competent cells showed that resistance genes of three strains of E.coli to streptomycin,lincomycin,metronidazole,ampicillin,amoxicillin,spectinomycin and amikacin,JX-22 and JX-26 isolates to doxycycline,florfenicol and cotrimoxazole,JX-22 isolate to ceftriaxone,and ceftriaxone,cefotaxime and norfloxacin genes of JX-28 isolate were located on a bacterial plasmid.Resistant to doxycycline,florfenicol and cotrimoxazole genes of JX-28 isolate,norfloxacin gene of JX-22 isolate and ceftriaxone and cefotaxime genes of JX-26 isolate were all located on their chromosomes.None of the three isolates had the ofloxacin resistance gene.In this experiment,most of the multidrug resistance genes of three multi-drug resistant E.coli were preliminarily located on the plasmid,which laid a foundation for further study on the drug resistance mechanism and effective control measures of E.coli.

Key words: E.coli; mult-drug resistance; drug resistance gene; drug sensitive test

CLC Number:

S852.61

YANG Xing, YIN Guihu, ZHANG Wenbo, TANG Yunyun. Preliminary Location of Resistant Genes of Three Escherichia coli Strains from Swine[J]. , 2019, 46(4): 1244-1251.

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Preliminary Location of Resistant Genes of Three Escherichia coli Strains from Swine

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