表达Ⅰ型耐热肠毒素的重组大肠杆菌诱发仔猪小肠炎症的分子机制研究

doi:10.16431/j.cnki.1671-7236.2019.04.023

Abstract

Abstract:

This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin (STa) on jejunum inflammation of 7 days old piglets.Twenty-four 7 days old piglets were allotted to four treatments:Control group (artificial milk),STa group (artificial milk+2×10⁹ CFU E.coli LMG194-pBAD-STa),LMG194 group (artificial milk+2×10⁹ CFU E.coli LMG194),and K88 group (artificial milk+2×10⁹ CFU E.coli K88),six piglets each group.The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day.The results showed that compared with control group,the jejunum villi of STa and K88 groups were significantly atrophied and shortened with obvious damage or even falling off.Compared with control group,the concentrations of IL-10,IL-8 and IL-6 in the serum of STa,LMG194 and K88 groups were significantly increased (P<0.05),and the concentrations of iFABP in the blood and intestinal tract of LMG194 and K88 groups were significantly decreased (P<0.05);The concentrations of TNF-α in serum of STa and K88 groups were significantly decreased,the concentration of NF-κB was significantly increased (P<0.05);The concentration of NF-κB in LMG194 group was significantly decreased,and the concentration of TNF-α was significantly increased (P<0.05).Compared with control group,the expression levels of CCL2 and CXCL9 genes in STa and LMG194 groups were significantly up-regulated (P<0.05),and the expression level of VNN1 gene was significantly down-regulated (P<0.05),the expression level of IFN-γ in STa and LMG194 groups was significantly increased and decreased,respectively (P<0.05);The expression of CXCL9 and IFN-γ genes was significantly down-regulated in K88 group (P<0.05),and the expression level of VNN1 gene was significantly up-regulated (P>0.05).There was no significant difference in CCL2 gene expression level (P>0.05).These results suggested that recombinant E.coli expressing type Ⅰ heat-stable enterotoxin STa was almost as toxic as E.coli K88,which could cause damage to intestinal mucosa of 7-day-old piglets,induce serious inflammatory reaction,lead to diarrhea in piglets,and could be used as a candidate strain for piglet diarrhea model.

Key words: heat-stable enterotoxin (STa); recombinant E.coli; piglets; inflammation

CLC Number:

S858.28

LI Xueni, SHI Yutao, DU Linxiao, LYU Yang, MA Zhipeng, YI Dan, WANG Lei, ZHAO Di, HOU Yongqing, WU Tao. Study on Molecular Mechanism of Intestinal Inflammation in Piglets Induced by Recombinant Escherichia coli Expressing Type Ⅰ Heat-stable Enterotoxin[J]. , 2019, 46(4): 1151-1157.

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Study on Molecular Mechanism of Intestinal Inflammation in Piglets Induced by Recombinant Escherichia coli Expressing Type Ⅰ Heat-stable Enterotoxin

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