山羊皮下脓肿的病原分离与鉴定

doi:10.16431/j.cnki.1671-7236.2019.03.037

Abstract

Abstract:

To identify the main pathogens of goat subcutaneous abscess,a total of 19 abscess samples were collected from three goat farms in Lezhi county,Jintang county and Pengxi county in Sichuan province.The pathogens were detected and identified by bacterial isolation,biochemical test and specific PCR methods,and the drug sensitivity of the pathogens isolated was determined.The results showed that a total of 19 strains of 3 pathogens were isolated by PCR amplification method,and the isolation rate of Corynebacterium pseudotuberculos,Staphylococcus aureus and Arcanobacterium pyogenes was 57.9%(11/19),26.3% (5/19) and 15.8% (3/19),respectively.A total of 17 strains of 3 pathogens were isolated by isolation and identification method,in which 11 strains of Gram-positive Brevibacterium were identified as Corynebacterium pseudotuberculosis whose isolation rate was 57.9%(11/19),4 strains of Gram-positive cocci were identified as Staphylococcus aureus,and isolation rate was 21.1% (4/19),while 2 strains of Gram-positive bacilli were identified as Arcanobacterium pyogenes whose isolation rate was 10.5% (2/19).PCR was more sensitive than bacterial isolation.Three pathogens were sensitive to many drugs such as amikacin and florfenicol,etc.The results of this study provided a useful basis for further analysis of its biological characteristics and the development of comprehensive control measures for subcutaneous abscess in these areas.

Key words: goat; subcutaneous abscess; isolation and identification; Corynebacterium pseudotuberculosis; Staphylococcus aureus; Arcanobacterium pyogenes

CLC Number:

S852.6

ZHANG Siqi, DAO Xiaofang, LI Yao, MA Yuan, YANG Falong. Isolation and Identification of Etiological Bacteria of Subcutaneous Abscess in Goats[J]. , 2019, 46(3): 949-956.

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Isolation and Identification of Etiological Bacteria of Subcutaneous Abscess in Goats

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