高产纤维素酶菌株的筛选、鉴定及产酶特性研究

doi:10.16431/j.cnki.1671-7236.2019.03.009

Abstract

Abstract:

In order to isolate cellulose-producing strain from soil samples of decayed leaf,the screening and identification of a cellulase-producing bacterium and its cellulase characterization were measured in this study.Firstly,the cellulose-producing strain was preliminarily isolated by carboxymethyl cellulose sodium (CMA-Na) culture medium,and then a high-yield cellulase strain B17 was obtained by enzymatic rescreening.Based on morphological observation and 16S rDNA sequencing,B17 was identified as Bacillus subtilis,and then it's fermentation conditions and enzymatic properties were preliminarily explored.The results showed that the optimal fermentation conditions for the cellulose-producting strain B17 were that the cultivate time,temperature and pH were 3 d,35℃ and 6.0.And under this condition,the maximum CMC activity and FPA activity were 85.48 and 59.85 U/mL,respectively.According to the results of enzymatic properties,the optimal temperature and pH for the enzyme were 50℃ and 6.0. Enzyme stability studies showed that enzyme kept active when the temperature was 30 to 60℃ and pH ranged from 4.0 to 7.0.In conclusion,a Bacillus subtilis with high cellulose activity had been isolated and identified,and it had high value for the feed's production of crop straw.

Key words: cellulose; Bacillus subtilis; screening; identification

CLC Number:

Q936

LI Hao, BAI Guangjian, LAN Nan, XU Jing, ZHAO Xingxiu, ZOU Wei. Screening and Identification of A Cellulase-producing Bacterium and Analysis of Its Cellulase Characterization[J]. , 2019, 46(3): 711-718.

References

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Screening and Identification of A Cellulase-producing Bacterium and Analysis of Its Cellulase Characterization

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