16味蒙药对金黄色葡萄球菌和大肠杆菌O8的体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2018.12.037

Abstract

Abstract:

In order to screen the Mongolian medicine which had good anti-bacterial effect on pathogenic Escherichia coli O8 (E.coli O8) and Staphylococcus aureus (S.aureus) in vitro,Oxford cup method was used to determine the inhibition zone diameter,microdilution borth method combine plate method was adopted to investigate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Mongolian medicine,and the fractional inhibitory concent ration index (FICI) of the sensitive Mongolian medicine against E.coli O8 and S.aureus was determined by chessboard method.The results showed that the inhibition zone diameter of six Mongolian medicines to S.aureus,such as Cortex Phellodendri,Lonicera japonica,Munronia henryi Harms,Schisandra chinensis,Herba Taraxaci and Cortex Fraxini,was 10.22 to 22.44 mm and MIC was 15.6 to 250 mg/mL.The inhibition zone diameter of four Mongolian medicines to E.coli O8,such as Schisandra chinensis,Lonicera japonica,Munronia henryi Harms and Cortex Fraxini,was 11.29 to 26.41 mm and MIC was 15.6 to 500 mg/mL.The FICI of Cortex Phellodendri combined Munronia henryi Harms to S.aureus was 0.36,while that of Munronia henryi Harms combined with Sanguisorba officinalis L.to E.coli O8 was 0.48.The comprehensive bacteriostatic effects of the Cortex Phellodendri,Lonicera japonica,Munronia henryi Harms,Schisandra chinensis,Herba Taraxaci and Cortex Fraxini were extremely sensitive or highly sensitive to S.aureus.The comprehensive antibacterial effect of Schisandra chinensis,Lonicera japonica,Munronia henryi Harms and Cortex Fraxini were extremely sensitive or highly sensitive to E.coli O8.Cortex Phellodendri,Munronia henryi Harms and Cortex Fraxini were the first the choice in the group of inhibiting S.aureus,Munronia henryi Harms and Sanguisorba officinalis L. were the first the choice in the group of inhibiting E.coli O8.

Key words: Mongolian medicine; Staphylococcus aureus; Escherichia coli O8; MIC; MBC; FICI

CLC Number:

S858.23

GAO Ruijuan, WANG Chunjie, AORIGELE, CHEN Yujie. Antibacterial Effects of Sixteen Mongolian Medicines Against Pathogenic Staphylococcus aureus and Escherichia coli O8 in vitro[J]. , 2018, 45(12): 3614-3622.

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Antibacterial Effects of Sixteen Mongolian Medicines Against Pathogenic Staphylococcus aureus and Escherichia coli O8 in vitro

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