2016—2017年广西牛呼吸道疾病综合征病原学的调查研究

doi:10.16431/j.cnki.1671-7236.2018.12.027

Abstract

Abstract:

To understand the etiology situation of bovine respiratory disease complex (BRDC) in Guangxi,117 samples with respiratory diseases during 2016 to 2017 were diagnosed by RT-PCR/PCR method and bacterial isolation and identification method.And the antibiotic susceptibility test of the main isolates were carried out.The results showed that detection rates of Klebsiella pneumoniae,Mycoplasma bovis,Trueperella pyogenes,Pasteurella multocida,IBRV and Mannheimia haemolytica were 41.0%(48/117),28.2%(33/117),20.5%(24/117),15.4%(18/117),12.8%(15/117) and 5.1%(6/117) detected by RT-PCR/PCR method,while the detection rates of Klebsiella pneumoniae,Escherichia coli,Mycoplasma bovis,Trueperella pyogenes,Pasteurella multocida and Mannheimia haemolytica by bactorial isolation and identification method were 41.0%(48/117),33.3%(39/117),17.1%(20/117),7.7%(9/117),2.6%(3/117) and 2.6%(3/117),indicating the former had the better sensibility,and BPIV3,BRSV and BVDV had not been detected.The results of antibiotic susceptibility tests showed that resistance rates of 20 Mycoplasma bovis strains against β-lactams,sulfonamides and peptides were from 90.0% to 100.0%,and they were sensitive to quinolones,gentamicin,amikacin and kanamycin (resistance rates were 10.0% to 25.0%).30 Escherichia coli strains and 30 Klebsiella pneumoniae strains were sensitive to cefotaxime,ceftriaxone and ceftazidime (13.3% to 20.0%,10.0% to 30.0%,respectively),and they were resistant to other 15 kinds of drugs (50.0% to 100.0%,40.0% to 100.0%,respectively).The results indicated that mixed infection was dominant in bovine respiratory disease in Guangxi caused by Mycoplasma bovis,Escherichia coli and Klebsiella pneumoniae which had developed different degrees of drug resistance.

Key words: bovine respiratory disease complex (BRDC); etiology investigation; Guangxi; drug resistance analysis

CLC Number:

S852.65

WU Cuilan, PENG Hao, LI Jun, LI Changting, TAO Li, WANG Qiuhua, PAN Yan, HAN Linmei, CHEN Zexiang, ZENG Yun. Research on Etiology of Bovine Respiratory Disease Complex in Guangxi During 2016 to 2017[J]. , 2018, 45(12): 3535-3544.

References

[1] SHOEMAKE B M,VANDER L B L,NEWCOMER B W,et al.Efficacy of oral administration of sodium iodide to prevent bovine respiratory disease complex[J].Journal of Veterinary Internal Medicine,2018,32(1):516-524.
[2] KISHIMOTO M,TSUCHIAKA S,RAHPAYA S S,et al.Development of a one-run Real-time PCR detection system for pathogens associated with bovine respiratory disease complex[J].Journal of Veterinary Internal Medicine,2017,79(3):517-523.
[3] JOHNSON K K,PENDELL D L.Market impacts of reducing the prevalence of bovine respiratory disease in United States beef cattle feedlots[J].Frontiers in Veterinary Science,2017,4:189.
[4] 范颖,张继瑜,周绪正,等.牛呼吸道疾病的病原学与防制研究进展[J].中国畜牧兽医,2013,40(1):157-163. FAN Y,ZHANG J Y,ZHOU X Z,et al.Research progress of the etiology and prevention of bovine respiratory disease[J].China Animal Husbandry & Veterinary Medicine,2013,40(1):157-163. (in Chinese)
[5] 马春霞,李军,覃勇,等.牛呼吸道疾病综合征病例的病原分析[J].中国畜牧兽医,2015,42(9):2481-2486. MA C X,LI J,QIN Y,et al.Pathogen analysis of bovine respiratory disease complex[J].China Animal Husbandry & Veterinary Medicine,2015,42(9):2481-2486.(in Chinese)
[6] 陶立,潘艳,李军,等.广西首例牛肺炎支原体的分离和鉴定[J].中国兽医杂志,2016,52(2):46-48. TAO L,PAN Y,LI J,et al.First isolation and identification of Mycoplasma pneumoniae in Guangxi[J].Chinese Journal of Veterinary Medicine,2016,52(2):46-48.(in Chinese)
[7] 陶立,李军,庞观龙,等.广西南宁市疑似牛支原体肺炎的病原分离和PCR鉴定[J].黑龙江畜牧兽医,2015,8:169-171. TAO L,LI J,PANG G L,et al.Pathogen isolation and PCR identification of suspected Mycoplasma pneumonia in Nanning,Guangxi[J].Heilongjiang Animal Science and Veterinary Medicine,2015,8:169-171.(in Chinese)
[8] 刘晓丹.6种牛源致病性细菌多重PCR检测方法的建立[D].哈尔滨:东北农业大学,2013. LIU X D.Development of multiplex PCR for detection of pathogenic bacteria[D].Harbin:Northeast Agricultural University,2013.(in Chinese)
[9] 于新友,李天芝,沈志强.牛病毒性腹泻病毒一步RT-PCR检测方法的建立及应用[J].中国奶牛,2015,9:27-29. YU X Y,LI T Z,SHEN Z Q.Establishment and application of one-step RT-PCR assay for BVDV detection[J].China Dairy Cattle,2015,9:27-29.(in Chinese)
[10] 王红,朴范泽,侯喜林.牛呼吸道合胞体病毒RT-PCR检测方法的建立[J].动物医学进展,2008,29(11):1-4. WANG H,PIAO F Z,HOU X L.Establishment of RT-PCR for detection of bovine respiratory syncytial virus[J].Progress in Veterinary Medicine,2008,29(11):1-4.(in Chinese)
[11] 黄张玲.广西部分地区牛场主要疫病血清流行病学调查[D].南宁:广西大学,2016. HUANG Z L.The seroepidemiological of major diseases in cattle farms in parts of Guangxi province[D].Nanning:Guangxi University,2016.(in Chinese)
[12] 苏娇秀,梁晟,曹慧慧,等.广西奶牛场主要疫病血清流行病学调查[J].中国兽医杂志,2016,52(5):52-53. SU J X,LIANG S,CAO H H,et al.The investigation of major epidemic diseases in dairy farms in Guangxi[J].Chinese Journal of Veterinary Medicine,2016,52(5):52-53.(in Chinese)
[13] 黄敬斌,吴翠兰,李军,等.一例牛肺炎克雷伯氏菌和大肠杆菌混合感染的诊断报告[J].广西畜牧兽医,2017,33(6):307-308. HUANG J B,WU C L,LI J,et al.Diagnostic report of a mixed infection of Klebsiella pneumoniae and Escherichia coli[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2017,33(6):307-308.(in Chinese)
[14] 胡长敏,石磊,龚瑞,等.牛支原体病研究进展[J].动物医学进展,2009,30(8):73-77. HU C M,SHI L,GONG R,et al.Research progress of Mycoplasma bovis[J].Progress in Veterinary Medicine,2009,30(8):73-77.(in Chinese)
[15] SULYOK K M,KREIZINGER Z,FEKETE L,et al.Antibiotic susceptibility profiles of Mycoplasma bovis strains isolated from cattle in Hungary,Central Europe[J].BMC Veterinary Research,2014,10:256.
[16] GAUTIER-BOUCHARDON A V,FERRE S,GRAND D L,et al.Overall decrease in the susceptibility of Mycoplasma bovis to antimicrobials over the past 30 years in France[J].PLoS One,2014,9(2):e87672.
[17] LERNER U,AMRAMA E,ROGER D,et al.Acquired resistance to the 16-membered macrolides tylosin and tilmicosin by Mycoplasma bovis[J].Veterinary Microbiology,2014,168(2-4):365-371.
[18] 贺丹丹,黄良宗,陈孝杰,等.不同动物源大肠杆菌的耐药性调查[J].中国畜牧兽医,2013,40(10):211-215. HE D D,HUANG L Z,CHEN X J,et al.Investigation of antibiotic resistance among Escherichia coli isolated from different animals[J].China Animal Husbandry & Veterinary Medicine,2013,40(10):211-215.(in Chinese)
[19] 冯娜,高翔,肖敏,等.牛源肺炎克雷伯氏菌的分离鉴定及遗传进化树分析[J].中国兽医科学,2016,46(11):1358-1364. FENG N,GAO X,XIAO M,et al.Isolation,identification and genetic evolution analysis of Klebsiella pneumon strain from calves[J].Chinese Veterinary Science,2016,46(11):1358-1364.(in Chinese)
[20] 马越,李景云,张新妹,等.1999~2002年北京、广东、湖北和辽宁地区大肠埃希氏菌及肺炎克雷伯氏菌临床分离株耐药性比较分析[J].中国抗生素杂志,2004,29(4):226-233. MA Y,LI J Y,ZHANG X M,et al.Study on antimicrobial resistance of Escherichia coli and Klebsiella pneumoniae isolates collected from hospitals in Beijing,Guangdong,Hubei and Liaoning regions from the year 1999 to 2002[J].Chinese Journal of Antibiotics,2004,29(4):226-233. (in Chinese)

Research on Etiology of Bovine Respiratory Disease Complex in Guangxi During 2016 to 2017

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Ting, LU Shengsheng, YANG Lili, LI Tianbao, ZHANG Min, JIANG Mingsheng. Based on Transcriptome Sequencing to Explore the Relationship Between Growth and Development Genes of Guangxi Ma Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3901-3908.
[2]	LYU Qiao, ZOU Chaoxia, WU Yongmei, ZHANG Ruimen, ZHENG Zihua, SHI Deshun, WEI Yingming, DENG Yanfei. Study on Regulation of STAT3 Gene on Myogenic Differentiation of Muscle Stem Cells in Guangxi Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2771-2777.
[3]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.
[4]	FENG Lingli, SI Jinglei, MO Jiayuan, LIU Xiaoxiao, TIAN Weilong, CHENG Feng, LI Yueyue, GUO Yafen, LIANG Jing, LAN Ganqiu. Cloning,Bioinformatics and Tissue Expression Analysis of BMP2 Gene in Guangxi Bama Mini Pig [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2302-2312.
[5]	ZHANG Xiyi, WEN Guilan, CHEN Guang, TIAN Lang, YANG Baiqi. Isolation,Identification and Drug Resistance Analysis of a Strain of Acinetobacter schindleri from Lohmann Pink Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2304-2311.
[6]	FENG Lingli, ZHANG Ruimen, HUANG Ye, XIA Panjie, ZHANG Mingyuan, LIANG Jing, LAN Ganqiu. Cloning and Eukaryotic Expression Vector Construction of MyoD1 Gene in Guangxi Bama Mini-pig [J]. , 2019, 46(4): 977-985.
[7]	XU Jia-ming, ZHANG Zheng-fen, HUA Guo-hong, YU Hui, HUANG De-chun, ZHAO Hai-quan, LI Hua. Study on Slaughter Performance and Meat Quality of Guangxi Partridge Chickens [J]. , 2017, 44(11): 3201-3207.
[8]	YANG Cui, HU Ting-jun, YANG Jian, HUANG Hong-ye, TAN Hong-lian, PENG Jian-bo, LUO Wen-juan, LI Lu, LI Chun-jie, LIAO Yu-ying, YANG Jia-huang. Effect of Dietary Picriafel-tarrae Lour Extracts on Serum Biochemical Indexes and Immune Function of Guangxi Partridge Chickens [J]. , 2017, 44(1): 65-73.
[9]	LI Ke-yu, ZHAO Wu, LI Bin, QIN Yi-bin, LU Bing-xia, LIANG Jia-xing, HE Ying, ZHOU Ying-ning, WEI Ying-yi. Cloning and Sequence Analysis of P97 Gene R1 Region of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig [J]. , 2015, 42(9): 2270-2277.
[10]	MA Chun-xia, LI Jun, QIN Yong, ZHONG Shu-hong, TAO Li, PENG Hao, LAN Mei-yi, YANG Wei, CHEN Ze-xiang, HU Shuai, LI Chang-ting, ZHAO Xiao-hong. Pathogen Analysis of Bovine Respiratory Disease Complex [J]. , 2015, 42(9): 2481-2486.
[11]	LI Yan-jun, SONG Shao-rui, GUO Ya-fen, LAN Gan-qiu, CHEN Bao-jian, YAN Xue-yu, WU Yan-jun, JIANG Qin-yang. Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig [J]. , 2015, 42(7): 1770-1776.
[12]	ZHANG Bing, LIAO Hai-hong, NONG Su-qun, MA Qing-yan, SUN Jun-li. The Genetic Diversity and Phylogenetic Relationships of Five Guangxi Pig Breeds and Three Exotic Pig Breeds by Analysis of mtDNA D-loop Sequences [J]. , 2015, 42(7): 1807-1815.
[13]	HUANG Jian-fang, LI Xiao-kai, JIANG Qin-yang, GUO Ya-fen, LAN Gan-qiu. Genetic Structure Analysis of Inbreeding Line in Guangxi Bama Miniature Pig [J]. , 2015, 42(2): 426-431.
[14]	WANG Ying, LI Xin-nan, HAN Juan-zhu, LIU Ying-zi, WANG Hong-jun, GAO Shen-yang, WU Si-si, ZHANG Li-bo, ZHOU Tie-zhong. Tracking Detection and Analysis of Drug Resistance of Chicken-derived Escherichia coli in a Broiler Farm [J]. , 2015, 42(12): 3329-3337.
[15]	LI Ying-ying,ZHAO Wu,LI Bin,QIN Yi-bin,LU Bing-xia,LIANG Jia-xing,HE Ying,ZHOU Ying-ning,LU Qin-zhang. Cloning and Sequence Analysis of P46 Gene of Mycoplasma hyopneumoniae in Guangxi Luchuan Pig [J]. , 2014, 41(2): 1-5.