广西巴马小型猪BMP2基因克隆、生物信息学及组织表达分析

doi:10.16431/j.cnki.1671-7236.2021.07.004

Abstract

Abstract: This study was aimed to clone BMP2 gene of Guangxi Bama Mini pigs and conduct bioinformatics analysis such as structure and function prediction,and analyze the difference in BMP2 gene expression between Guangxi Bama Mini pigs and Landrace pigs.The genomic DNA of Guangxi Bama Mini pigs was used as template and single nucleotide polymorphism (SNPs) in the exon region of BMP2 gene were identified by sequencing.Then,using variety of bioinformatics software to carry out a series of analysis on the promoter region,coding region and 3'UTR region of BMP2 gene in Guangxi Bama Mini pigs.The basic physical and chemical properties,phosphorylation site,O-glycosylation site,transmembrane region,hydrophilicity and hydrophobicity,protein modification structure,and secondary and tertiary structure of BMP2 protein in Guangxi Bama Mini pigs were predicted and analyzed.The miRNA binding sites and related pathways were analyzed,and the tissue expression profiles of Guangxi Bama Mini pigs and Landrace pigs were drawn.The results showed that the CDS region of BMP2 gene in Guangxi Bama Mini pigs was 1 188 bp in length,encoding 395 amino acids.Compared with pig reference genome BMP2 gene of Guangxi Bama Mini pigs had synonymous mutations in G1663A and G1897C,three missense mutations in C1896T (Pro→Leu),T1971C (Gly→Ser) and G2000A (Leu→Ser),and 13 miRNA binding regions in 3'UTR region,including miR-142-5p and miR-129-5p.The homology of Guangxi Bama Mini pig with Sus scrofa,Bos taurus,Bubalus bubals,Capra hircus,Ovis aries,Equus cabalus,Canis lupus dingo,Canis lupus familiaris and Macaca mulatta was 99.92%,91.75%,91.84%,91.92%,91.92%,90.50%,89.43%,90.91% and 90.91%,respectively.The results of phylogenetic tree showed that Guangxi Bama Mini pigs had the highest similarity with Sus scrofa,had a relatively close genetic distance with Bos taurus,and had a relatively distant genetic relationship with Macaca mulatta.The molecular weight of BMP2 protein was 44.55 ku,and the theoretical isoelectric point was 8.37.It was predicted that there were 12 O-glycosylation sites and 42 phosphorylation sites.The prediction analysis showed that BMP2 protein was hydrophilic and was an extracellular protein.Tissue expression profile showed that the expression of BMP2 gene in growth plate cartilage of Guangxi Bama Mini pigs was significantly lower than Landrace pigs (P<0.05).This study provided a theoretical basis for further exploring the function of BMP2 gene in Guangxi Bama Mini pigs,and also provided an important reference for the utilization of Guangxi Bama Mini pigs breed resources.

Key words: Guangxi Bama Mini pigs; BMP2 gene; bone; bioinformatics analysis

CLC Number:

S828

FENG Lingli, SI Jinglei, MO Jiayuan, LIU Xiaoxiao, TIAN Weilong, CHENG Feng, LI Yueyue, GUO Yafen, LIANG Jing, LAN Ganqiu. Cloning,Bioinformatics and Tissue Expression Analysis of BMP2 Gene in Guangxi Bama Mini Pig[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2302-2312.

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Cloning,Bioinformatics and Tissue Expression Analysis of BMP2 Gene in Guangxi Bama Mini Pig

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