不同猪种ADIPOQ基因外显子2多态性及其与山西白猪体重和体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.12.023

Abstract

Abstract:

This study was aimed to investigate the polymorphism of adiponectin (ADIPOQ) gene exon 2 and its correlation on body weights and body sizes in Shanxi White pig.A total of 392 pigs from Landrace pig,Large White pig,Duroc pig,Shanxi White pig,Shanxi Black pig and Mashen pig populations were used to detect the polymorphism of ADIPOQ gene exon 2 by PCR-SSCP,and the association of the SNPs on body weights and body sizes in Shanxi White pig was analyzed by GLM.The results showed that there was a missense mutation of G→A at 89 bp of ADIPOQ gene exon 2,which resulted in the change of amino acid from valine (Val) to isoleucine (Ile).There were three genotypes (AA,AB and BB) and two alleles (A and B) at this site.There was only BB genotype in Duroc pig,and the advantageous genotype was also BB genotype in Landrace pig,Large White pig,Shanxi White pig and Shanxi Black pig,while AA genotype frequency in Mashen pig was greater than AB and BB genotypes.In the imported pig breeds such as Landrace pig,Large White pig and Duroc pig,the frequency of the dominant allele B were 0.96,0.96,and 1.00,respectively.In Chinese domestic Mashen pig,the frequency of allele A (0.52) was little greater than that of allele B (0.48).The frequency of allele B in Shanxi White pig and Shanxi Black pig were 0.76 and 0.78,respectively.The genotype distribution among Mashen pig,Shanxi White pig and Shanxi Black pig was not significant(P>0.05),there was no significant difference between Duroc pig and Landrace pig or Duroc pig and Large White pig (P>0.05),however,the difference of genotype distribution between Landrace pig and Large White pig was significant (P<0.05).Comparing to the imported breeds with Chinese domestic Mashen pig and the composite populations of Shanxi White pig and Shanxi Black pig,the genotype distribution difference of each other was extremely significant (P<0.01).The effect of the SNP in exon 2 of ADIPOQ gene on weaning weight was significant,the weaning weight at 28-day-old of BB genotype individuals was significantly greater than those of AA and AB genotypes(P<0.05),and there was no significant difference between AA and AB genotypes(P>0.05).The effect of this polymorphic site on other traits was not significant,which indicated that it mainly played role at early stage of pig growth and development.

Key words: pig; ADIPOQ gene; polymorphism; body weight traits; body measurement traits

CLC Number:

S813.3

ZHOU Shenghua, ZHAO Yuanyuan, WANG Yuanyuan, WANG Hejie, QIN Benyuan, ZHANG Ningfang, LE Baoyu, CHENG Zhimin, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Polymorphism of Adiponectin Gene Exon 2 in Different Pig Breeds and Its Relationship with Body Weights and Body Measurements in Shanxi White Pig[J]. , 2018, 45(12): 3497-3504.

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Polymorphism of Adiponectin Gene Exon 2 in Different Pig Breeds and Its Relationship with Body Weights and Body Measurements in Shanxi White Pig

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