脂多糖对胚胎附植期Toll样受体4及相关免疫因子的影响

doi:10.16431/j.cnki.1671-7236.2018.11.025

Abstract

Abstract:

The aim of this study was to investigate the effect of LPS on the expression of TLR4 and related immune factors in embryo implantation stage of sheep.The constructed pcDNA3.1-TLR4 overexpression vector was used to transfect sheep endometrial stromal cells,and Western blotting was used to identify the transfection effect.Then the endometrial stromal cells were stimulated with 1 μg/L LPS,and the inflammatory model of endometrial stromal cells was established.The TLR4 and related immune factors IL-1β and IL-6 of cell treated with LPS were detected after cultured for 12,24,48 and 72 h by Real-time quantitative PCR and Western blotting methods with untreated cell as the control.The results showed that compared with the control group,LPS promoted the release of IL-1β and IL-6,but the expression of IL-6 in the cells gradually decreased with time,and the expression of IL-1β increased gradually,which made Th1/Th2 biased to the Th1 direction.The expressions of TLR4 mRNA at 12,24 and 72 h were all significantly higher than that in control group (P<0.05),and the expression at 48 h was extremely significantly higher than that in control group (P<0.01),and the expression of TLR4 protein after LPS treatment was always higher than that of the control group.In conclusion,the pcDNA3.1-TLR4 overexpression vector was successfully transferred into the endometrial stromal cells of sheep.LPS effectively activated the TLR4 signaling pathway in endometrial cells and promoted the expression of downstream factors.The TLR4 protein in the decidua tissue also played an important role in maintaining the balance of early pregnancy microenvironment.

Key words: pcDNA3.1-TLR4; TLR4; stromal cells; LPS

CLC Number:

S814

KONG Weihuan, DAI Rong, WAN Pengcheng, SHI Guoqing. Effects of LPS on TLR4 and Related Immune Factors During Embryo Implantation Stage[J]. , 2018, 45(11): 3185-3191.

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Effects of LPS on TLR4 and Related Immune Factors During Embryo Implantation Stage

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