鼠源重组UBC13蛋白对脂多糖诱导的小鼠急性炎症的影响

doi:10.16431/j.cnki.1671-7236.2019.04.024

Abstract

Abstract:

To investigate the effect of murine recombinant UBC13 protein on lipopolysaccharide (LPS)-induced acute inflammation in mice,twenty-four SPF female mice were randomly divided into 4 groups:PBS group,LPS model group,UBC13 high and low dose groups (100 and 25 μg per mouse,respectively),with 6 mice in each group.LPS model group and each protein dose group were intraperitoneally injected with 20 mg/kg LPS,and the PBS group was intraperitoneally injected with equal volume of PBS.After 1 h of injection,each protein group was injected subcutaneously with recombinant UBC13 protein at the corresponding dose,PBS group and LPS model group were injected equal volume PBS.The mice were sacrificed 24 h after the administration of the protein.The lung,spleen,thymus and liver tissues of the mice were collected,the organ indexes were calculated.The histopathological changes were observed by HE staining and the relative expression of IL-1β, TNF-α and IL-6 mRNA in lung,spleen,thymus and liver and iNOS mRNA in lung were detected by Real-time PCR.The results showed that compared with PBS control group,the indexes of lung,spleen and liver in LPS model group were significantly or extremely increased (P<0.05; P<0.01),and the lung,spleen,thymus and liver tissues showed pathological changes.The results of Real-time PCR showed that the relative expressions of IL-1β,TNF-α and IL-6 mRNA in lung,spleen,thymus and liver of LPS model group were extremely significantly increased compared with PBS control group (P<0.01).The relative expression of iNOS mRNA in lung was also extremely significantly increased (P<0.01).Compared with LPS model group,the pathological changes in lung,spleen and liver were significantly improved in high dose group of UBC13 protein;The expressions of IL-1β,TNF-α and IL-6 mRNA in lung,liver and spleen were extremely significantly decreased (P<0.01),and the expression of iNOS mRNA in lungs was also extremely significantly decreased (P<0.01);The expression of TNF-α mRNA in thymus was significantly decreased (P<0.05),the expression of IL-6 mRNA and IL-1β were extremely significantly decreased (P<0.01).It indicated that murine recombinant UBC13 protein could down-regulate the expression of inflammatory factors and alleviate LPS-induced acute inflammatory response in mice.

Key words: UBC13 protein; LPS; acute inflammation; inflammatory factor

CLC Number:

R392

HAN Rong, WANG Chun, TAO Jiali, REN Lulu, GUO Yin, JIANG Junbing. Effect of Murine Recombinant UBC13 Protein on Acute Inflammation Induced by LPS in Mice[J]. , 2019, 46(4): 1158-1165.

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Effect of Murine Recombinant UBC13 Protein on Acute Inflammation Induced by LPS in Mice

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