三丁酸甘油酯对乙酸刺激仔猪肠道屏障功能的影响

doi:10.16431/j.cnki.1671-7236.2018.09.012

Abstract

Abstract:

This study was conducted to study the effect of tributyrin (TB) on small intestine mucosal growth and gut barrier function of acetic acid (ACA)-treated piglets.Eighteen crossbred healthy piglets (Ducoc×Landrace×Yorkshire,28 days old) were randomly allocated into three treatment groups (control,ACA and TB).The pigs in control and ACA groups were fed basal diet,and TB group was fed the basal diet supplemented with 0.1% TB.On day 15 of the trial,ACA and TB groups were intracolonically administrated with 10 mL of 10% ACA solution,control group were given sterile saline at the same volume.On days 18 and 21,blood samples were collected from jugular vein for the determination of DAO activity.On day 21 of the trial,piglets were killed and the samples of small intestinal mucosa were collected for the measuring of intestinal morphological structure and mucosal injury correlated genes mRNA levels.The results showed that:①Before ACA administration,comparing to control group,dietary supplementation with TB could increase ADG,but the difference was not significant (P=0.089),while reduced F/G significantly (P<0.05);②Compared with control group,ACA administration significantly decreased the villus height and surface area of villus in the jejunum,and the ratio of villus height to crypt depth in the ileum (P<0.05);③Compared with ACA group,TB supplementation significantly decreased the plasma DAO activity (P<0.05),and decreased the expression of AREG gene in the ileum mucosa extreme significantly (P<0.01).Collectively,dietary supplementation with 0.1% TB could alleviate ACA-induced damages on mucosal growth and barrier function in the small intestine of piglets.

Key words: tributyrin; acetic acid; piglets; gut barrier

CLC Number:

Q493.99

ZHANG Junmei, SONG Zhuan, ZHOU Ying, LIU Wenkai, YI Dan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Tributyrin on Gut Barrier Function of Acetic Acid-treated Piglets[J]. , 2018, 45(9): 2440-2446.

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Effects of Tributyrin on Gut Barrier Function of Acetic Acid-treated Piglets

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