蛋白激酶C抑制剂及激活剂对鸭肠炎病毒增殖的影响

doi:10.16431/j.cnki.1671-7236.2018.07.034

Abstract

Abstract:

To identify the pathogenesis of duck enteritis virus (DEV),inhibitor and activator of PKC (SP and PMA) were used to research that whether PKC/PKCI could affect the proliferation of DEV,trying to provide some new ideas for understanding the mechanism of DEV infection.Normal duck fibroblasts(DEF) were treated with SP or PMA,and Real-time quantitative PCR method was used to measure the expression levels of PKC and PKCI genes at different time.The DEV was used to infect DEF which treated with SP or PMA,then the cell cultures were collected at different time,and the TCID₅₀ and expression levels of DEV NP gene were measured by Reed-Muench and Real-time quantitative PCR,respectively.The results showed that there was no significant effect of SP treatment on PKC/PKCI genes expression levels in DEF (P>0.05),while the PMA treatment could extremely significantly increased the PKC gene expression (P<0.01),but had no significant impact on PKCI gene (P>0.05);SP/PMA processing could significant or extremely significant affect the ability of DEV replication (P<0.05;P<0.01),and the expression level of DEV NP gene was significantly or extremely significant decreased at the early stage of infection (P<0.05;P<0.01).The above results indicated that the effects of SP and PMA on PKC/PKCI were different,while both of them could effectively inhibit the proliferation of DEV.These results could provide the basis for DEV prevention and its pathogenesis studying.

Key words: duck enteritis virus (DEV); protein kinase C (PKC); PKC inhibitor; PKC activator; proliferation

CLC Number:

S853.65

HE Xinwei, GOU Wanli, ZHANG Mingyang, ZHOU Bijun, CHENG Zhentao, WEN Ming. Effects of Protein Kinase C Inhibitor and Activator on Proliferation of Duck Enteritis Virus[J]. , 2018, 45(7): 2001-2007.

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Effects of Protein Kinase C Inhibitor and Activator on Proliferation of Duck Enteritis Virus

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