肿节风三清颗粒抗炎和抗哮喘作用

doi:10.16431/j.cnki.1671-7236.2018.07.035

Abstract

Abstract:

The study was aimed to investigate the anti-inflammatory and antiasthmatic effects of Zhongjiefeng Sanqing granules.The cell viability that treated with 200,400,800 and 1 600 μg/mL Zhongjiefeng Sanqing granules was measured by MTT method.The inflammation model of RAW264.7 cell was established with LPS in vitro,and then TNF-α and IL-6 levels in the cell supernatant were measured by ELISA after 400,800 and 1 600 μg/mL Zhongjiefeng Sanqing granules treatment.The asthma model established by sensitizing and challenging female BALB/c mice with ovalbumin (OVA) to evaluate the effects of Zhongjiefeng Sanqing granules on asthmatic mice in vivo, the airway hyperresponsiveness (AHR) was measured at 24 h after the final OVA challenge, and the bronchoalveolar lavage fluid (BALF) were harvested to measure the number of inflammatory cells and the concentrations of IL-4,IL-5 and IL-13, and the serum was collected to measure the level of OVA-IgE.The results showed that Zhongjiefeng Sanqing granules in the concentration range of 200 to 1 600 μg/mL could significantly or extremely significantly promote the proliferation of RAW 264.7 cell (P<0.05;P<0.01).Compared with the LPS group,800 and 1 600 μg/mL Zhongjiefeng Sanqing granules could extremely significantly reduce the levels of TNF-α and IL-6 (P<0.01).Compared with the asthma model group, Zhongjiefeng Sanqing granules obviously ameliorated the airway hyperresponsiveness,markedly reduced the number of inflammatory cells in BALF,concentrations of IL-4, IL-5 and IL-13 in BALF and level of OVA-IgE in serum.These results suggested that Zhongjiefeng Sanqing granules had protective effect on inflammatory cell model and asthmatic model of mouse by inhibiting the expression of inflammatory cytokines.

Key words: Zhongjiefeng Sanqing granules; anti-inflammation; antiasthma; inflammatory factors

CLC Number:

R931.6

LIANG Zhengmin, LI Jinhui, CUI Yumei, PENG Jianbo, TAO Qing, HE Jiakang. Anti-inflammatory and Antiasthmatic Activities of Zhongjiefeng Sanqing Granules[J]. , 2018, 45(7): 2008-2014.

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Anti-inflammatory and Antiasthmatic Activities of Zhongjiefeng Sanqing Granules

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