复方忍冬藤提取物促进骨折愈合及抗炎作用研究

doi:10.16431/j.cnki.1671-7236.2017.01.039

Abstract

Abstract:

This experiment was aimed to study the effects of mixed Caulis Lonicerae japonicae extracts on fracture healing and inflammation. Rabbit right radius fracture model was induced by dental drill in order to study the medicine's effect on fracture healing. Moreover,the contents of Ca,P and alkaline phosphatase in serum were measured to explore the influences of mixed Caulis Lonicerae japonicae extracts on serum biochemical indexes of fractured rabbits.RAW264.7 cell was induced by LPS in order to research the effect of medicine on inflammation. The concentrations of TNF-α,IL-1β,IL-6 and NO were measured by ELISA. The results showed that the rabbit fracture model was successfully prepared by dental drill, and the extracts of Caulis Lonicerae japonicae could increase the levels of Ca and alkaline phosphatase and reduce the P content in the serum of fractured rabbits. The TNF-α,IL-1β and NO levels in RAW264.7 cell induced by LPS were significantly inhibited by the extracts with concentrations ranging from 50 to 200 μg/mL (P<0.05). However, the extracts had no significant effect on the level of IL-6 (P>0.05). These results demonstrated that the extracts of mixed Caulis Lonicerae japonicae could promote the fracture healing and possessed good resistance inflammation effect.

Key words: mixed Caulis Lonicerae japonicae; extracts; fracture healing; anti-inflammation

CLC Number:

S858.291

JIA Hai-yan, TENG Xu-dong, WANG Hui, ZHANG Hui-zhen. Effects of Mixed Caulis Lonicerae japonicae Extracts on Fracture Healing and Anti-inflammation[J]. , 2017, 44(1): 275-281.

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Effects of Mixed Caulis Lonicerae japonicae Extracts on Fracture Healing and Anti-inflammation

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