梅山猪CD14基因多态性及其对部分免疫指标和繁殖性能的关联分析

doi:10.16431/j.cnki.1671-7236.2018.07.021

Abstract

Abstract:

This study was aimed to investigate the genetic effect of cluster of differentiation antigen 14 (CD14) gene polymorphism on partial immune indexes and reproductive performance in Meishan pigs.The genetic polymorphism of CD14 gene -61 bp locus in Meishan pigs was detected by PCR-RFLP method,the level of the partial vital cytokines (IL-1β,IL-4,IL-6,IL-8,IL-10,IFN-γ,TNF-α and TGF-β1) and reproductive performances (total litter size,number of weaning piglets,birth litter weight and weaning litter weight) were determined,and the association of CD14 gene polymorphism with these immune indexes and reproductive performance was analyzed.The results showed that the -61 bp locus of CD14 gene in Meishan pigs was digested by BamH Ⅰ,and three genotypes were detected named as AA,AG and GG,respectively.The Chi-square test results showed that the genotype distribution of Meishan pigs were in Hardy-Weinberg equilibrium (P>0.05),the polymorphism information content (PIC) were moderate polymorphism in Meishan pigs.The correlation analysis indicated that the level of IL-10 of AG and GG genotypes individuals was significantly higher than that of GG genotype individuals (P<0.05).There was no significant difference of reproductive performances among three genotypes in primiparous sow (P>0.05),while the weaning litter weight of AA and AG genotypes individuals was significantly higher than that of GG genotype individuals in multiparous sows (P<0.05).The results of this experiment showed that GG genotype might be unfavorable genotype both in general disease resistance and reproductive performance when Meishan pigs were molecularly selected based on the -61 bp locus of CD14 gene,and the -61 bp locus of CD14 gene could be used as a potential genetic marker for further systematic verification and analysis.

Key words: pig; CD14 gene; immune indexes; reproductive performance

CLC Number:

S813.3

DU Yulu, WEI Zongyou, YE Lan, BAO Wenbin, WU Shenglong. Polymorphism of CD14 Gene and Its Association with Partial Immune Indexes and Reproductive Performance in Meishan Pigs[J]. , 2018, 45(7): 1898-1904.

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Polymorphism of CD14 Gene and Its Association with Partial Immune Indexes and Reproductive Performance in Meishan Pigs

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