贵州中蜂蜂房哈夫尼菌的分离鉴定及药敏试验

doi:10.16431/j.cnki.1671-7236.2018.04.027

Abstract

Abstract:

For the diagnosis,prevention and treatment of Apis cerana's bacterial disease in Guizhou,a pathogen was identified in this paper by isolation and purification,morphological observation and biochemical test.The bacterial 16S rRNA gene universal primers were used for amplification and sequencing.The results of the sequencing were analyzed on NCBI.16S rRNA sequences of 30 bacteria of 5 genera with high homology were selected to analyze the nucleotide homology and construct the phylogenetic tree,and animal regression test and drug sensitivity test of the isolate were carried out.The results showed that the isolate was short gram-negative bacilli,and the biochemical tests were initially identified as Hafnia alvei.16S rRNA sequences shared the highest homology with Hafnia,ranging from 97.0% to 99.6%.The phylogenetic tree indicated that the isolate belonged to the branch of Hafnia.The animal regression test showed that the isolate had some pathogenicity to Apis cerana,indicating that the bees were infected by Hafnia alvei.Antibiotic sensitive test showed that the isolate was resistant to penicillin,erythromycin and clindamycin,and was sensitive to amikacin,sulfamethoxazole and ofloxacin.The results of this study provided a reference for the study of honeybee Hafnia infection in Apis cerana and provided reasonable drug advice for the bee farms.

Key words: Apis cerana; Hafnia alvei; 16S rRNA; isolation and identification

CLC Number:

S891

QIU Jiyu, ZHU Xing, ZHONG Chunyan, LUO Yayan, HOU Ping, FAN Ying, FAN Wensui, DENG Weixi, HAN Jianlun. Isolation, Identification and Drug Sensitive Test for Hafnia alvei from Apis cerana in Guizhou[J]. , 2018, 45(4): 1050-1058.

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Isolation, Identification and Drug Sensitive Test for Hafnia alvei from Apis cerana in Guizhou

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