某定点肉牛屠宰场中非O157致病性STEC的分离鉴定

doi:10.16431/j.cnki.1671-7236.2018.04.025

Abstract

Abstract:

This study was aimed to understand the contamination of Escherichia coli at the process of beef cattle slaughter in the region of Yili,Xinjiang,and to detect the infection of non-O157 pathogenic Shiga toxin-producing E.coli (STEC).The fecal samples of beef cattle and swab samples of carcass surface in a certain designated slaughterhouse in Yili were collected,and the samples were performed with an isolated identification of E.coli,PCR detection of virulence genes (eae,stx1 and stx2), O157 identification (rfbE),ERIC-PCR genotyping and mouse pathogenicity test.The results showed that 42 strains of E.coli were isolated and identified out from 45 samples,and the isolation rate was 93.3%.Among them, the virulence gene of eae was not detected,and two E.coli strains simultaneously programmed the virulence genes of stx1 and stx2,and the detection rate was 4.8%.All the identified strains were non-O157 type.Genotyping test of ERIC-PCR revealed that the two strains of non-O157 STEC were very similar and closely related to each other.The mice were injected with intraperitoneal injection,and started to die after 6 h of bacterial attack.It showed that there was a presence of intestinal hemorrhage,its liver,spleen and kidney were found to have obvious hemorrhage and enlargement,meanwhile the anatomical control mice showed no sign of anomaly,indicating that the strains had a certain pathogenicity.In conclusion,E.coli contamination was exist in the process of slaughtering of beef cattle.The non-O157 STEC in the faeces had contaminated the carcass,and the environmental health of key link of slaughter and processing of beef cattle should be strengthened.

Key words: non-O157 STEC; isolation and identification; virulence gene; ERIC-PCR

CLC Number:

S852.61

ZHANG Yan, LIU Yingyu, ZHENG Xiaoqin, ZHANG Chao, SU Zhanqiang, ZHANG Xiaohong. Isolation and Identification of the Pathogenic Non-O157 STEC in Beef Slaughterhouse[J]. , 2018, 45(4): 1035-1040.

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Isolation and Identification of the Pathogenic Non-O157 STEC in Beef Slaughterhouse

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