水貂志贺氏菌分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2017.11.038

Abstract

Abstract:

In order to provide therapeutical guidance for drug admistration, the bacteria of three sick minks suffering from typical diarrhea symptoms provided by mink farms in Jilin province were isolated and identified, and the drug sensitivity was tested. The bacteria were isolated with TSA plates, and identified using biochemical methods and PCR assay. The virulence of the isolates was determined by infecting BALB/c mice. The antimicrobial susceptibility of the isolates to antimicrobial agents was investigated using the K-B method. PCR was used to detect the resistance genes and Ⅰ integrons. A total of 3 Shigella isolates were obtained from sick minks. The virulent determination showed that all isolates could cause mice diarrhea. The drug sensitivity results showed that 3 strains were sensitive to fluoroquinolone, cephalosporin, florfenicol and polymyxin, but they were resistant to aminoglycoside, tetracycline, chloramphenicol, penicillin and ampicillin. There were seven resistance genes were detected,bla_TEM-1,aadA1, aac(3')-Ⅱc, aac(6')-Ⅰb, aph(3')-Ⅶ, tet(M), cat2 and three class Ⅰ integrons carrying aadA 1 gene cassette. All of the isolates were virulent and caused the mice diarrhea. The resistance of the 3 strains were very serious and mainly for multiple drug resistance phenomenon. The resistance genes detected in the mink were various, and could bring enormous implications for clinical treatment.

Key words: mink; Shigella; diarrhea; drug sensitivity

CLC Number:

S858.92

SUN Na, CHEN Qiang, YANG Yan-ling, GUO Li, FENG Qiu-ju, SUN Ming, CHENG Shi-peng. Isolation, Identification and Drug Sensitivity Analysis of Shigella from Minks[J]. , 2017, 44(11): 3379-3384.

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Isolation, Identification and Drug Sensitivity Analysis of Shigella from Minks

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