禽霍乱巴氏杆菌的分离鉴定及基因分型

doi:10.16431/j.cnki.1671-7236.2017.09.026

Abstract

Abstract:

In order to determine the suspected avian cholera pathogen and geneotypes, bacteria isolation technology was used to isolate and culture pathogenic bacteria, the isolated bacteria were identified by traditional methods and molecular biological methods. Geneotyping of the isolated bacteria were determined using PCR technology and gene sequence analysis. The results demonstrated that the isolated bacteria had typical culture characteristics of Pasteurella multocida (Pm),and the morphology of the colony, the characteristics of cell staining, the physiological and biochemical characteristics were consist with Pm; 457 bp fragment was amplified by PCR. Geneotyping results showed that only A primer amplified the target gene fragment of 1 050 bp, sequence analysis also showed that the isolated bacteria shared 97.6% to 100.0% sequence homology with reference strains, and phylogenetic tree analysis showed that the isolated bacteria and A type Pm were in a branch.The experimental results indicated that the isolated bacteria was identified as capsular serotype A of Pm, the results provided reference for the prevention and control of fowl cholera.

Key words: fowl cholera; Pasteurella multocida; isolation and identification; genotyping

CLC Number:

S852.61

TANG Sha, YUAN Hai-wen, YANG Yuan, ZHANG Yun-dan, WANG Jun, CHENG Zhen-tao, TANG Ying-xiu, CHEN Bo. Isolation, Identification and Genotyping of Pasteurella multocida from Fowl Cholera[J]. , 2017, 44(9): 2724-2730.

References

[1] 陆承平.兽医微生物学[M].北京:中国农业出版社,2001.
[2] Carter G R. Studies on Pasteurella multocida. Ⅰ. A hemagglutination test for the identification of serological types[J]. American Journal of Veterinary Research, 1955, 16(60):481-484.
[3] Heddleston K L, Gallagher J E, Rebers P A. Fowl cholera:Gel diffusion precipitin test for serotyping Pasteurella multocida from avian species[J]. Avian Diseases, 1972, 16(4):925-936.
[4] Townsend K M, Boyce J D, Chung J Y, et al. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system[J]. Journal of Clinical Microbiology, 2001, 39(3):924-929.
[5] Christensen H, Kuhnert P, Busse H J, et al. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae[J]. International Journal of Systematic & Evolutionary Microbiology, 2007, 57(1):166-178.
[6] 黄海燕,王印, 彭娟,等. 猪源多杀性巴氏杆菌PCR鉴定方法的建立[J]. 畜牧兽医学报, 2012, 43(7):1111-1116.
[7] Townsend K M, Frost A J, Lee C W, et al. Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates[J]. Journal of Clinical Microbiology, 1998, 36(4):1096-1100.
[8] Kalorey D R, Yuvaraj S, Vanjari S S, et al. PCR analysis of Pasteurella multocida isolates from an outbreak of pasteurellosis in Indian pigs[J]. Comparative Immunology Microbiology & Infectious Diseases, 2008, 31(6):459-465.
[9] 杨发龙,张焕容,夏梦芸,等.禽A型多杀性巴氏杆菌的分离和鉴定[J].四川畜牧兽医,2008,211(5):20-23.
[10] 李永明,罗阿东,徐景峨,等.猪源多杀性巴氏杆菌的生物学鉴定与荚膜PCR分型[J].中国预防兽医学报,2007,29(7):506-509.
[11] 马光强, 刘丽娟, 龙丹丹,等. 畜禽源3种革兰氏阴性杆菌多重PCR检测方法的建立与应用[J]. 中国畜牧兽医, 2016, 43(3):577-584.
[12] 张东超, 杨宁宁, 龙雨清,等. 肉鸡禽呼肠孤病毒与巴氏杆菌混合感染的鉴别诊断[J]. 中国畜牧兽医, 2015, 42(12):3364-3370.
[13] 彭忠,梁婉,吴斌.多杀性巴氏杆菌分子分型方法简述[J].微生物学报,2016, 56(10):1521-1529.
[14] 李伟杰,魏财文,田野,等. 猪源多杀性巴氏杆菌荚膜分型及外膜蛋白H基因序列分析[J]. 中国畜牧兽医, 2015, 42(1):32-37.
[15] 张媛, 魏财文, 李建,等. 多重PCR在多杀性巴氏杆菌荚膜定群中的应用[J]. 中国兽医科学, 2014,12:1286-1291.
[16] 刘吉山, 姚春阳, 莫玲,等. 长毛兔巴氏杆菌病诊断与防控[J]. 中国畜牧兽医, 2014, 41(12):282-286.
[17] 杜慧慧, 潘婷婷, 伍晨露,等. 牛源多杀性巴氏杆菌新型交叉保护性抗原的筛选[J]. 中国兽医学报, 2016, 36(8):1293-1300.
[18] 赵战勤, 刘倩玉, 席晓剑,等. 猪源A型和D型多杀性巴氏杆菌强毒菌株的生物学特性比较研究[J]. 中国预防兽医学报, 2016, 38(5):366-370.
[19] 何传雨,丁国婵,王静梅,等. 不同牛源多杀性巴氏杆菌HasR基因的克隆及序列分析[J]. 中国畜牧兽医, 2011, 38(8):56-59.
[20] 高明燕, 徐步, 龚建森,等. 禽多杀性巴氏杆菌血清型与外膜蛋白型相关性研究[J].黑龙江畜牧兽医, 2011,15:18-21.

Isolation, Identification and Genotyping of Pasteurella multocida from Fowl Cholera

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