奶牛原代乳腺上皮细胞的培养及β酪蛋白mRNA的表达

doi:10.16431/j.cnki.1671-7236.2017.07.011

Abstract

Abstract:

The aim of this study was to construct the culture method of primary mammary epithelial cells in vitro using mammary tissue and to test expression of β-casein mRNA in mammary gland epithelial cells. The fresh lactating mammary gland tissue was obtained, microscope observation was used for cell morphology and cell growth counting,and the Real-time PCR method was used for detection of β-casein mRNA expression. The results showed that purified primary mammary gland epithelial cells formed island-like and grew with typical shape of slabstone and cobblestone; Primary mammary gland epithelial cells growth curve was "S" type and accorded with rule of general cell growth. Mammary epithelial cells expressed β-casein mRNA successfully. In conclusion, the primary mammary epithelial cells with normal physiology function had been established successfully. The study provided favourable cell model for further research of the functions of mammary epithelial cells.

Key words: dairy cow; primary; mammary epithelial cell

CLC Number:

S823.9⁺1

PU Jun-hua, ZHU Xiao-rui, XU Xin, LU Xu-bin, MAO Yong-jiang, YANG Zhang-ping. Culture of Dairy Cow Primary Mammary Epithelial Cells and Expression of β-casein mRNA[J]. , 2017, 44(7): 1975-1981.

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Culture of Dairy Cow Primary Mammary Epithelial Cells and Expression of β-casein mRNA

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