Bcl-xL及PTD-FNK蛋白的细胞保护作用研究进展

doi:10.16431/j.cnki.1671-7236.2017.07.010

Abstract

Abstract:

In this article,firstly,the structure and functions of Bcl-xL protein is briefly introduced. It is believed that Bcl-xL protein as a member of the Bcl-2 family,has the same head folding structures as Bcl-2 family,which plays an important role in suppressing apoptosis of cell and protecting various cells from injury. Then the author introduces the biological properties,functions, mechanisms and clinical application of PTD-FNK protein (a gain-of-function mutant of Bcl-xL). It is thought that PTD-FNK protein is able to rapidly penetrate into cell in vitro and in vivo, and can protect various types of cells from many damage through the inhibition of apoptosis in the path of mitochondrion,so being a potent protein with various therapeutic applications. Finally,the author points out that the difficulty of mass production and practical application of two proteins is that prokaryotic expression of the proteins is inclusion body,which need the complex refolding process in order to obtain soluble proteins,and proteins' loss in the refolding process is great. The priorities and direction of future research are to realize the soluble expression and purify the two proteins in large quantities by optimizing expression conditions of induction,laying the foundation of their application to clinical medicine and cell preservation under room temperature, low temperature and frozen condition.

Key words: Bcl-xL protein; PTD-FNK protein; cytoprotection

CLC Number:

WANG Ying-qun, SHI Bo-mei, LI Xun, XU Chun-rong, LI Mei-zhen, TANG Rong-fu, WANG Xiao-ye, HU Chuan-huo. Research Progress on Cytoprotection of Bcl-xL and PTD-FNK Protein[J]. , 2017, 44(7): 1967-1974.

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Research Progress on Cytoprotection of Bcl-xL and PTD-FNK Protein

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