LDL对玻璃化冷冻解冻培养MⅡ期猪卵母细胞线粒体膜电位和透明带泛素化水平的影响

doi:10.16431/j.cnki.1671-7236.2017.07.009

Abstract

Abstract:

The purpose of this study was to determine the effect of LDL addition on oocyte developmental rate,mitochondrial membrane potential and ZP protein ubiquitination level in MⅡ oocytes from vitrified-warmed GV oocytes.Mitochondrial membrane potentials were labeled with specific probe JC-1,and ZP protein samples of different treatment groups in MⅡ oocytes were analyzed by SDS-PAGE and Western blotting methods. The results showed that normal rate and in vitro maturation of MⅡ oocytes in the 10 mg/mL LDL group (71.92% and 69.86%) were significantly higher than those in control group (58.26% and 54.55%)(P<0.05),that were nearest to non-vitrified group.The mitochondrial membrane potential of MⅡ oocytes in the 10 mg/mL LDL group was significantly higher than those in control group and 1,20 mg/mL LDL groups (P<0.05).The ubiquitinated ZP1,ZP2 and ZP3 of MⅡ oocytes in non-vitrified group and LDL treated groups were labeled at 61,80 and 106 ku,respectively.The ubiquitinated ZPs (ZP1,ZP2 and ZP3) level of MⅡ oocytes in the 10 mg/mL LDL group was significantly higher than those in 1 and 20 mg/mL LDL groups (P<0.05),while significantly lower than that of non-vitrified group (P<0.05). In conlusion, LDL could improve the maturation rate, mitochondrial membrane potential and ubiquitinated ZPs level of MⅡ oocytes from vitrified-warmed GV oocytes.

Key words: oocytes; vitrification; low density lipoprotein (LDL); mitochondrial membrane potential; ZP protein ubiquitination

CLC Number:

S814

JIANG Ze, XUAN Biao, LI Zuo-chen, WANG Yi-nan, JIN Yi. Effect of LDL on the Mitochondrial Transmembrane Potential and ZP Protein Ubiquitination in Vitrified-warmed MⅡ Oocytes[J]. , 2017, 44(7): 1961-1966.

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Effect of LDL on the Mitochondrial Transmembrane Potential and ZP Protein Ubiquitination in Vitrified-warmed MⅡ Oocytes

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