一株凝结芽孢杆菌的分离鉴定及益生性研究

doi:10.16431/j.cnki.1671-7236.2017.04.036

Abstract

Abstract:

This experiment was conducted to isolate and identify a strain of Bacillus coagulans from the soil at the bottom of the pond, and study its probiotic properties. A strong acid producing bacillus was isolated from the soil at the bottom of the pond and identified as Bacillus coagulans through the physiological and biochemical characteristics and 16S rDNA sequence analysis. The strain had high tolerance to the simulated gastric juice (pH 2.5, 12 h of incubation, survival rate was 71.9%) and simulated intestinal fluid (bile salt concentration 0.3%, 12 h of incubation, survival rate was 84.4%) and had high antibacterial activity to E.coliK88, E.coliK99, S.agalactiaeand A. hydrophila. It could degrade nitrite effectively and the degradation rate was 96.80% in 48 h. Two hundred and seventy AA broilers aged 1 day were randomly allocated into 3 groups. Diets were added 0 (group Ⅰ), 1.0×10⁶ (group Ⅱ) and 1.0×10⁷ CFU/g (group Ⅲ) Bacillus coagulans NJ102, respectively. The experiment lasted for 42 d. The average daily gain and final body weight of group Ⅱ and Ⅲ were significantly higher than that of group Ⅰ(P<0.05), and the feed weight ratio of the two groups were significantly lower than that of groupⅠ(P<0.05). There was no significant difference in average feed intake among three groups (P>0.05). It was found that the soil-derived Bacillus coagulans NJ102 from the bottom of the pond had a certain degree of resistance and potential probiotic ability, which could improve the growth performance of broilers and had the potential to be a microbial feed additive.

Key words: Bacillus coagulans; isolation; identification; bottom soil; probiotic; broiler

CLC Number:

S816.7

WANG Pan, YI Gan-feng, SUN Zi-bo, WANG Rui, CHEN Li-xian, XIA Dong-mei. Study on the Isolation，Identification and Probiotic Characteristics of a Bacillus coagulans[J]. , 2017, 44(4): 1195-1202.

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Study on the Isolation，Identification and Probiotic Characteristics of a Bacillus coagulans

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