免疫磁珠富集法对牛源大肠杆菌O157∶H7分离鉴定的影响

doi:10.16431/j.cnki.1671-7236.2017.04.035

Abstract

Abstract:

In order to prove the effect of immunomagnetic beads adsorption technology on separation efficiency of bovine E.coli O157∶H7 in practice,a total of 18 fecal samples, 162 anal samples,10 fodder samples,17 water samples and 36 samples of carcass surface cotton were collected from three cattle farms in Wujiaqu,Yining and Changji. The samples were taken by EC bead enrichment and direct SMAC and MUG test,respectively. Then,the bacterial rfbE gene and flagellarfliC gene were detected by PCR. Finally,the suspected E.coli O157∶H7 bacteria were detected by biochemical test. 8 and 4 strains of E.coliO157∶H7 were isolated from 243 samples,respectively. There was no statistical difference between the two methods. This experiment showed that the immunomagnetic beads adsorption technique in practice was not statistically significant compared with the conventional method, but it did increase the number of isolated E.coli O157∶H7,which was consistent with previous research results.

Key words: immunomagnetic beads; E.coli O157∶H7; isolation; identification; bovine

CLC Number:

S852.61

DING Hao, SU Zhan-qiang, XIA Li-ning, WANG Yu-meng, WANG Dong, ZHANG Jin-yu, ZHANG Tao, KUANG Ling, YAO Gang. Influence of Immunomagnetic Enrichment Method for Isolation and Identification of E.coli O157∶H7 from Bovine[J]. , 2017, 44(4): 1189-1194.

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Influence of Immunomagnetic Enrichment Method for Isolation and Identification of E.coli O157∶H7 from Bovine

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