赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.04.025

Abstract

Abstract:

This study was aimed to analyze the association of myostatin (MSTN) gene polymorphisms with meat quality traits in Chishui Black-bone chicken. The DNA pooling was constructed and the direct sequencing technology of PCR product was used to detect the single nucleotide polymorphism (SNP) of MSTN gene. The results showed that C324T were detected in exon 1 of MSTN gene in Chishui Black-bone chicken. However, there was not found SNP site in exon 2 and exon 3 of MSTN gene. The C324T site was devised into three genotypes of CC, CT and TT. The association relationships between the genotype of MSTN gene and meat quality traits revealed that chest muscle pH and leg muscle pH value of Chishui Black-bone chicken with CT genotype were significantly higher than that of CC and TT genotypes (P<0.05), the shear force of CT genotype was significantly higher than that of TT genotype (P<0.05), there was no significant difference in other meat quality traits (P>0.05). This results suggested that the pH value and shear force of MSTN gene had a larger genetic effect in Black-bone chicken,MSTN gene was one of genes which could control the preliminary inference, might affect the pH value and shear force or with the main gene chain in Black-bone chicken, and could be used as pH value and shear force of molecular markers of Black-bone chicken.

Key words: Chishui Black-bone chicken; MSTN gene; meat quality

CLC Number:

S813.3

LI Wei, ZU Pan-yu, LI Hong-lin, LIN Jia-dong, ZHANG Fu-ping. Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken[J]. , 2017, 44(4): 1115-1121.

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Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken

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