一株产角蛋白酶的铜绿假单胞菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2017.03.041

Abstract

Abstract:

A new feather-degrading bacterium was isolated from soil samples colected from pile-up feather places in this study.The transparent circle was produced after the strain was cultured on the milk medium for 24 h,the colony shape of the strain was irregular,rough surface,edge blur,and could produce green fluorescent pigment.It was gram negative,short rod,nospore and capsule and had a flagellum scanning by electron microscope. Biochemical test showed that the isolate could hydrolyze milk and gelatin,utilize citrate with keratinase activity and nitrate reduction reaction positive.Phylogenetic tree analysis showed that the isolate was most closely related to Pseudomonas aeruginosa strain (GenBank accession No.:BAMA01000316).Combined with the above results,the isolated strain was identified as Pseudomonas aeruginosa B1-2. When it was inoculated on basal medium containing 1% feather as sole carbon and nitrogen source,the highest level of keratinase activity was 60.3 U/mL after 48 h while the degradation rate of feather was 85.7%. This result indicated that the newly isolated Pseudomonas aeruginosa B1-2 could be useful in management of farm wastes.

Key words: isolation and identification; biochemical test; keratinase; Pseudomonas aeruginosa

CLC Number:

X172

HUANG Miao-rong, ZHONG Chu-hong, REN Guang-cai, LIU Chuan-gao, YE Jun-xian, CHEN Rui-ai. Isolation and Identification of A Keratinase-producing Strain of Pseudomonas aeruginosa B1-2[J]. , 2017, 44(3): 920-927.

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Isolation and Identification of A Keratinase-producing Strain of Pseudomonas aeruginosa B1-2

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