不同地区鸭疫里氏杆菌的分离鉴定与药敏试验

doi:10.16431/j.cnki.1671-7236.2017.01.034

Abstract

Abstract:

In order to understand the prevalence of the duck infectious serositis in different regions,and further to determine the sensitivity of the commonly used drugs for the Riemerella anatipestifer in these area.Bacteria were isolated from suspected infected ducks in Yunfu, and Kaiping in Guangdong province and Changshan in Zhejiang province.The isolated bacteria were identified by differential culture, microscopic examination and biochemical test. Micro dilution method was carried out in drug sensitivity test, and the MIC values of the isolated strains to the commonly used drugs were determined.As a result, a total of 16 strains of Riemerella anatipestifer were isolated and identified from three areas. Data of the MIC showed that all isolates were highly sensitive to cephalosporins and florfenicol;Doxycycline andspectinomycin were sensitive too,but enrofloxacin, neomycin and other tested drugs showed different degrees of resistance.The present study provided a systematic method for the isolation and identification of the Riemerella anatipestifer and for the drug sensitivity test.At the same time, it also provided theoretical guidance for the rational drug control of the duck infectious serositis.

Key words: Riemerella anatipestifer; isolation; identification; drug-sensitivity test; MIC

CLC Number:

S852.61⁺2

YONG Yan, LIU Guo-chang, FANG Bing-hu, QIAO Yan-hong. Isolation, Identification and Drug-sensitivity Test of Riemerella anatipestifer from Different Areas[J]. , 2017, 44(1): 242-247.

References

[1] 程安春,朱德康,王晓佳,等.鸭疫里默氏杆菌病原及其防控关键技术研究[J].中国家禽,2012,34(6):1-4.
[2] 程安春,汪铭书,陈孝跃,等.我国鸭疫里氏杆菌血清型调查及新血清型的发现和病原特性[J].中国兽医学报, 2003,23(4):320-323.
[3] 蔡秀磊,韦强,崔言顺,等.鸭疫里默氏杆菌临床分离株的耐药性与Ⅰ型整合子检测[J].中国预防兽医学报,2007,29(9):727-731.
[4] 孙静,郭浩,王秀玲,等.近年我国部分地区鸭疫里默氏杆菌耐药性分析[J].山东畜牧兽医,2011,32(5):61-62.
[5] 李振清.山东地区鸭疫里氏杆菌的分离鉴定[J].畜牧与兽医,2012,44(6):59-62.
[6] Yu C Y, Liu Y W, Chou S J, et al. Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms[J].Avian Pathology, 2008, 37(3):273-279.
[7] Zhou Z,Peng X,Xiao Y, et al. Genome sequence of poultry pathogen Riemerella anatipestifer strain RA-YM[J].Journal of Bacteriology,2011,193(5):1284-1285.
[8] Mavromatis K,Lu M, Misra M, et al.Complete genome sequence of Riemerella anatipestifer type strain (ATCC 11845T)[J].Standards in Genomic Sciences,2011,4(2):144-153.
[9] 钟登科,魏建超,张训海,等.鸭疫里氏杆菌的分离鉴定及药敏试验[J].畜牧与兽医,2008,40(7):86-88.
[10] 印宝东.鸭传染性浆膜炎的病原分离培养及药敏试验[J].现代畜牧兽医,2012,6:58-59.
[11] CLSL.VET01-A4.Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals, Approved Standard, Fourth Edition[S].2013.
[12] 魏光河.渝西地区两株鸭疫里氏杆菌的分离鉴定与药敏试验[J].中国兽医科学,2015,45(5):481-485.
[13] 吴彩艳,覃宗华,袁建丰,等.广东地区鸭疫里氏杆菌的血清型及抗药性情况调查[J].畜牧与兽医,2009,41(5):22-25.
[14] 张桂荣.鸭疫里氏杆菌的分离鉴定及药物敏感性分析[J].黑龙江畜牧兽医,2012,7:125-127.
[15] 刘丽君,万培伟,万根,等.鸭疫里氏杆菌的分离鉴定和药敏试验[J].江西畜牧兽医杂志,2014,5:11-13.
[16] 李春芬,李郁,魏建忠,等.安徽省部分地区鸭疫里氏杆菌的分离鉴定及生物学特性研究[J].中国预防兽医学报,2009,31(3):197-200.
[17] 刁冬雪.鸭疫里氏杆菌的分离鉴定及耐药性的初步研究[D].南京:南京农业大学,2013.
[18] Chang C F, Lin W H, Yeh T M,et al. Antimicrobial susceptibility of Riemerellaanatipestifer isolated from ducks and the efficacy of ceftiofur treatment[J].J Vet Diagn Invest,2003,15(1):26-29.
[19] 张大丙,郭玉璞. 我国鸭疫里氏杆菌血清型的鉴定[J]. 畜牧兽医学报,1999,30(6):536-542.
[20] 李富祥,杨斌,常志顺,等.间接ELISA检测鸭疫里默氏杆菌血清抗体方法的建立[J].中国畜牧兽医,2010,37(1):202-206.
[21] 赵青松,汪铭书,程安春,等.鸭传染性浆膜炎母源抗体消长规律及其对后代雏鸭的保护[J].中国预防兽医学报,2016,38(2):120-123.

Isolation, Identification and Drug-sensitivity Test of Riemerella anatipestifer from Different Areas

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