奶牛乳房炎白色念珠菌的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2016.12.040

Abstract

Abstract:

The study was aimed to learn the infection of dairy cows with fungi in Ningxia and take effective measures to prevent and control the fungi infection. Milk samples were collected from dairy cows that did not respond to antibiotics in Ningxia, fungi in milk samples were separated and identified, and the biological characteristics of isolated bacteria were analyzed. Milk samples infected by fungus were screened out using fungal selective medium. The yeast pathogen was shown to be Candida albicans by biochemistry and molecular biology methods. Determination of enzyme activity and biofilm-forming tests confirmed that it had different levels of virulence with strong pathogenicity to experimental animals. Drug sensitive test was carried out by K-B programe, the results showed that the isolates were resistant to clinical commonly used antibiotics, and were sensitive to clinical common antifungal drugs. The fungal mastitis was caused by Candida albicans in Ningxia, the ratio of fungal infection was 43%. Candida albicans had strong virulence and were resistant to common antibiotic and sensitivity to antifungal drugs and traditional Chinese medicine.

Key words: Candida albicans; mastitis; pathogenicity; haemolysin

CLC Number:

S858.23

HE Fang, LU Jin-xia, FENG Feng, ZHOU Xue-zhang. Isolation, Identification and Analysis of Biological Characteristics of Candida albicans in Bouine Mastitis[J]. , 2016, 43(12): 3356-3362.

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Isolation, Identification and Analysis of Biological Characteristics of Candida albicans in Bouine Mastitis

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