蓖麻蛋白对人外周血B淋巴细胞的作用及相关基因表达的研究

doi:10.16431/j.cnki.1671-7236.2016.11.001

Abstract

Abstract:

This study was amied to learn the toxic effects of ricin on human peripheral blood B lymphocytes（IM-9）and the expression of related genes.The extracted ricin of different concentrations were added to culture cells for 6 and 12 h to make sure the median inhibitory concentration,then culture cells at the median inhibitory concentration for 2,6,10 and 12 h,at each time point detect the expression of immune related genes CD40,IL-1β,TNF-α and apoptosis related genes Bcl-2,Bax,Caspase-3.The results showed that the inhibitory effect of ricin on IM-9 cells was increased with the increase of concentration and time.The expression of TNF-α and CD40 genes increased significantly in experimental group than control group at 6 h（P<0.05).10 and 12 h reached extremely significant level（P<0.01);There was no significance of IL-1β gene expression between experimental and control groups（P>0.05).About apoptosis gene,the Bax gene expression decreased extremely significantly in experimental group at 10 h（P<0.05）and decreased significantly at 12 h（P<0.05);Bcl-2 gene had reached significant levels of four time periods（P<0.05), and 2,12 h were extremely significant（P<0.01);The expression of Caspase-3 reduced at 6 h,the other time points increased significantly（P<0.05),at 2 h reached extremely significant level（P<0.01).It showed that the gene expression of IM-9 cells could be significantly affected by ricin,TNF-α and CD40 genes were two potential genes to evaluate the toxicity by IM-9 cells.

Key words: ricin; peripheral blood B lymphocyte; gene expression

CLC Number:

ZHEN Er-dong, HUANG Su-juan, HUA Chao-ju, TAO Cong, YANG Shu-lin, WANG Yan-fang, ZHOU Rong, AO Hong, LI Kui. Study on the Effect of Ricin on Human Peripheral Blood B Lymphocyte and the Expression of Related Genes[J]. , 2016, 43(11): 2803-2810.

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Study on the Effect of Ricin on Human Peripheral Blood B Lymphocyte and the Expression of Related Genes

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