贵州矮马鼠伤寒沙门氏菌的分离鉴定及其致病力研究

doi:10.16431/j.cnki.1671-7236.2016.10.036

Abstract

Abstract:

To investigate the reason of the diarrhea in Guizhou pony,we used the feces of pony as experimental material to isolate and detect pathogenic bacteria.S6 strain was isolated from SS and XLD medium,and identified using Gram staining,biochemical tests and molecular phylogeny methods.The results showed that S6 strain could growth on SS and XLD medium,and the Gram staining was negative.Biochemical test suggested that its phenotype features were accordance with Salmonella.The 16S rRNA gene sequence of S6 strain was determined in a nucleotide sequence identity of 99% with Salmonella typhimurium ATCC 13311 (NR_119108).Based on the morphological and molecular phylogenetic results,the strain was identified as Salmonella typhimurium S6 strain.It was virulent to mice with the median lethal dose (LD₅₀) of 4.71×10² CFU.Then,we amplified the invasion protein A (invA) gene by PCR method.The invA gene isolated from S6 strain contained 6 to 8 bp different from the known gene,which resulted in only one amino acid substitution.The mutant sites of invA gene might attribute to the pathogenicity of S6 strain.The detection rate of Salmonella was 57% in Guizhou pony population.It was inferred that the diarrhea in Guizhou pony might be caused by virulent Salmonella typhimurium.

Key words: Guizhou pony; Salmonella; invasive protein A gene; pathogenicity

CLC Number:

S852.6

DENG Xian-you, XUE Yan, WANG Jia-fu, RAN Xue-qin. Isolation,Identification and Pathogenicity of Salmonella typhimurium from Guizhou Pony[J]. , 2016, 43(10): 2756-2761.

References

[1] 高雪.中国矮马遗传资源的研究[J].草食家畜,2000,12(4):7-13.
[2] 侯文通.我国矮马源流简析[J].农业考古,1990,1:340-343.
[3] Magdesian K G,Hirsh D C,Jang S S,et al.Characterization of Clostridium difficile isolates from foals with diarrhea:28 cases (1993-1997)[J]. Journal of the American Veterinary Medical Association,2002,220(1):67-73.
[4] Ward M P,Alinovi C A,Couetil L L,et al.A randomized clinical trial using probiotics to prevent Salmonella fecal shedding in hospitalized horses[J]. Journal of Equine Veterinary Science,2004,24(6):242-247.
[5] Parsons S K,Bull C M,Gordon D M.Low prevalence of Salmonella enterica in Australian wildlife[J].Environmental Microbiology Reports,2010,2(5):657-659.
[6] 金春.微生物学实验指导[M].北京:清华大学出版社,2005.
[7] 常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[8] Watson A J.Genesis of fat emboli[J].Journal of Clinical Pathology.Supplement (Royal College of Pathologists),1970,4:132.
[9] 顾兵,张政,李玉萍,等.半数致死量及其计算方法概述[J].中国职业医学,2009,36(6):507-508.
[10] 黄晓冬,蔡建秀,林海鸿.天竺桂挥发油的毒理学研究[J].时珍国医国药,2012,23(1):138-139.
[11] 周雷,沈勇.家畜腹泻的病因及诊断要点[J].养殖技术顾问,2013,9:128.
[12] 王俊红.山东省猪源致病性沙门氏菌的分离鉴定与耐药性分析[D].泰安:山东农业大学,2008.
[13] 刘振湘,曾元根,钟福生,等.鸵鸟鼠伤寒沙门氏菌的分离与鉴定[J].中国动物检疫,2001,18(6):25-26.
[14] 刘振相,钟福生.小灵猫鼠伤寒沙门氏菌的分离与鉴定[J].中国动物检疫,2001,18(11):25-26.
[15] 陈溥言.兽医传染病学[M].北京:中国农业出版社,2006.
[16] 郭树荣,李效芳.鼠伤寒沙门氏菌在外环境中的存活能力[J].中国消毒学杂志,1992,9(3):171-175.
[17] Ginocchio C C,Galan J E.Functional conservation among members of the Salmonella typhimurium invA family of proteins[J].Infection and Immunity,1995,63(2):729-732.
[18] Dvaid W,Jnae T,Sion P G.Detection of PCR products using self-probing amplicons and fluorescence[J].Nature Biotechnology,1999,17(5):804-807.

Isolation,Identification and Pathogenicity of Salmonella typhimurium from Guizhou Pony

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[2]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[3]	KANG Yuhui, ZHOU Hongshan, JIN Wenjie, QIN Aijian. Isolation, Identification and Pathogenicity of a Strain of Plesiomonas shigelloides from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3676-3682.
[4]	SHAO Mingzhu, ZHAO Yunqing, LI Songli, HOU Shaohua, ZHANG Yanying. Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3683-3691.
[5]	WANG Zhifang, XU Yindi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, LI Haili, WANG Keling. Analysis of Virulence and Drug Resistance of Haemophilus parasuis Serotype 7 Strains [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3245-3255.
[6]	GUO Qian, ZHANG Yu, FANG Xiaowei, AN Ni, YUAN Mei, FANG Chun. Study on Glycosyl-flippase gtcA Gene Deletion Reduces the Pathogenicity of Listeria monocytogenes Serotype 1/2a [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2298-2306.
[7]	WANG Simin, YANG Jiangfeng, ZHAO Xiaokun, ZHANG Lei, GUO Jingjing, LAN Jinping, YU Xiaojie, WANG Jing. Isolation, Identification and Analysis of Pathogenicity and Drug Resistance of Salmonella from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2385-2396.
[8]	DONG Hongyan, SONG Haigang, ZHU Shanyuan, YU Shengqing, XIE Jun, HONG Shuangpeng, GUO Changming, XU Hai, GAO Yuexiu. Isolation,Identification and Pathogenicity Analysis of Escherichia coli from Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1977-1984.
[9]	HOU Xiaohong, WANG Meng, WU Bo, LONG Xianrong, ZHAO Xiaomin, GAO Jing, DAI Peiqiang, CHAI Tongjie. Infectivity of H9N2 Subtype Avian Influenza Virus SD18 Strain and Comparison of the Effects of Different Routes of Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1449-1460.
[10]	LAN Hong, REN Guangcai, YE Junxian, XIONG Ting, HE Xianming, LIU Yufu, YANG Zekun, HAO Wenqian, CHEN Ruiai. Isolation, Identification and Pathogenicity of Fowl Adenovirus Group Ⅰ Serotype 11 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1067-1076.
[11]	CHEN Xiaofei, ZHANG Bin, ZHANG Chunhong, ZHANG Jianfeng, LIAO Ming. Isolation, Identification and Pathogenicity Analysis of Porcine Rotavirus Group A Strain SCJY-13 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 660-668.
[12]	ZHANG Haolei, SALANG Wenzhu, HUANG Zhihong, WEN Yongping, ZHANG Huanrong. Isolation, Identification, Drug Resistance and Pathogenicity Analysis of Salmonella from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4887-4897.
[13]	LI Yuqi, XU Liting, QUE Qunyan, LIAO Xin, ZHENG Xin, YUAN Qingyang, WU Linyue, XIE Xinjie, TIAN Ying, YANG Shoushen, LI Xiaohua, DAI Ailing, YANG Xiaoyan, FAN Kewei. Isolation,Identification and Pathogenicity of Enteroinvasive Escherichia coli from Porcupine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4372-4382.
[14]	CHEN Bingxun, HAN Dongmei, ZHAO Weiwei, ZHANG Bo, DENG Jing, WAN Quan, WANG Xi, XIAO Peng, LYU Longbao, GAO Hong. Isolation and Identification of Escherichia coli from Tupaia belangeri and Detection and Sequence Analysis of Genes Related to High Pathogenicity Island [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4466-4475.
[15]	XUE Xiaoyan, ZHANG Zhenxing, ZENG Yi, JI Jia, DAI Hongyang, LI Suhua, SONG Jianling. Isolation and Identification of Yunnan Fowl Adenovirus-4 and Sequence Analysis of Hexon Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3932-3942.