畜禽长链非编码RNA的功能及其研究方法

doi:10.16431/j.cnki.1671-7236.2016.09.030

Abstract

Abstract:

Long noncoding RNA (LncRNA) is a class of RNA molecules,which is more than 200 nt in length and not capable of encoding proteins.It mainly participates in various biological processes including lipid metabolism,muscle development,embryonic development,sex determination,differentiation and diseases control.The establishment and application of research methods plays a critical role in the research on LncRNA.The review introduces the biological characteristics and biological function of LncRNA in livestock and poultry,focusing on the research methods of LncRNA.Currently used methods in LncRNA research include gene chip,RNA-seq,Northern blotting,fluorescence in situ hybridization,Real-time quantitative PCR,parallel analysis of RNA structure (PARS) and fast predictions of RNA and protein interactions and domains (CatRAPID).In addition,the methods for analyzing the interaction of LncRNA with DNA,RNA,and protein including RNA-pulldown,RNA binding protein immunoprecipitation (RIP),photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation (PAR-CLIP),RNA antisense purifyication (RAP),chromatin isolation by RNA purification (ChIRP),capture hybridization analysis of RNA targets (CHART) and crosslinking,ligation and sequencing of hybrids (CLASH) are highlighted in order to provide a reference for research of LncRNA in livestock and poultry.

Key words: long noncoding RNA; livestock and poultry; biological function; research method

CLC Number:

LU Qi-rong, LIU Meng-ke, LI Li, CHENG Gu-yue, HAO Hai-hong, WANG Xu, DAI Meng-hong, YUAN Zong-hui. Function and Research Methods of Long Noncoding RNA in Livestock and Poultry[J]. , 2016, 43(9): 2425-2434.

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Function and Research Methods of Long Noncoding RNA in Livestock and Poultry

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