水貂阿留申病毒地方强毒株ADV-LN的分离鉴定

doi:10.16431/j.cnki.1671-7236.2016.06.030

Abstract

Abstract: Mink suspected infection aleutian mink disease virus (ADV) from mink breeding areas in Liaoning province were tested with CIEP method.The mink with antibody to ADV were selected and culled.Liver,spleen,kidney and mesenteric lymph node samples were taken for pathological examination and the viruses were observed under electron microscope.The grinded tissue fluid filter was added penicillin and treptomycin and inoculated into CRFK cells and passaged by 6 times for virus isolation.And cells cultures were identified as ADV by PCR.Then they were inoculated into healthy mink.Three days later,the mink showed clinical signs,which including the loss of appetite,anemia,hair dull,antifeedant and binge drinking.Some minks showed neurological symptoms,manifested symptoms of convulsions,cramps,staggering gait,ataxia,or hind limb paralysis and died.The virus strains isolated and identified were named as the ADV-LN.

Key words: aleutian mink disease virus; isolation; identification

CLC Number:

S852.65

JIA Yun, LI Yan-wu, SUN Ming-ying, ZHANG Xue, ZHANG Yan-yan, SONG Da-he, LIU Zhao, WANG Quan-kai, SUN Ying-jie. Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN[J]. , 2016, 43(6): 1597-1603.

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Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN

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