单核细胞增生李斯特氏菌实时荧光定量PCR快速检测方法的建立

doi:10.16431/j.cnki.1671-7236.2016.06.008

Abstract

Abstract: To establish a rapid assay for Listeria monocytogenes(LM) detection,a Real-time PCR method was developed targeting iap gene of LM.The results showed that the test for 15 bacteria strains,only LM was positive,indicated that the method had high specificity.In addition,the sensitivity of Real-time PCR was 6.5 CFU/mL.Stability and reproducibility of the test showed that the coefficient of variation for the same sample repeat the Ct values were less than 2%.Furthermore,a total of 3 positive samples for LM were detected from 139 clinical samples by the method,which was in accordance with the testing result by GB 478930-2010 standard detection protocol.Therefore,the Real-time PCR method provides a novel rapid,sensitive and good repeatability detection method for LM infection.

Key words: Listeria monocytogenes(LM); iap gene; Real-time PCR

CLC Number:

LI Dan-dan, XU Yi-gang, LI Meng-yuan, WANG Yu, QIU Suo-ping, GAO Hui-jiang, GAO Shen-yang. Development of A Dual Real-time PCR for the Rapid Detection of Listeria monocytogenes[J]. , 2016, 43(6): 1453-1457.

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Development of A Dual Real-time PCR for the Rapid Detection of Listeria monocytogenes

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