抑制大肠杆菌K88的益生菌体外筛选

doi:10.16431/j.cnki.1671-7236.2016.05.035

Abstract

Abstract: The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.

Key words: probiotics; Lactobacillus casei; Bacillus coagulans; IPEC-1 cell line; LDH activity; plate count method

CLC Number:

S852.6

WANG Lei, LV Yang, ZHANG Yue, XIE Jia-qian, ZHAO Di, YAN Li-qiong, MEI Hui-min, WANG Lei, YI Dan, CHEN Hong-bo, DING Bin-ying, HOU Yong-qing, WU Tao. Screening of Probiotics Inhibiting E. coli K88 in vitro[J]. , 2016, 43(5): 1355-1360.

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Screening of Probiotics Inhibiting E. coli K88 in vitro

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