奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2016.05.016

Abstract

Abstract: The aim of the study was to isolate and identify S.cerevisiae in the dairy feed and analyze antibacterial activity of S.cerevisiae fermentation brothe in vitro.5 forage samples and 5 feed samples were collected to isolate yeast by YPD solid medium.Biochemical identification and 26S rDNA sequence were used to identify the isolated strains.The antibacterial activity of S.cerevisiae strains was tested by cylinder plate method.A total of 12 yeast belonged to 4 species were isolated and identified, including 3 S.cerevisiae (Y1, Y7 and Y11), 4 Debaryomyces hansenii, 3 Cryptococcus flavescens and 2 Torulaspora debrueckii.The antibacterial activities of the 3 S.cerevisiae fermentation brothe to Staphylococcus aureus, E.coli and Streptococcus agalactiae were different.Y7 and Y11 fermentation brothe showed antibacterial activities to all the tested pathogens, while Y1 inhibit E.coli alone.This study laid the foundations for further research of probiotics containing S.cerevisiae with excellent performance.

Key words: S.cerevisiae; cows feed; isolation and identification; antibacterical activity

CLC Number:

S816

LIU Long-hai, LI Xin-pu, YANG Feng, LUO Jin-yin, ZHANG Zhe, LI Hong-sheng. The Study of Isolation, Identification of S.cerevisiae from Dairy Feed and its Antibacterical Activity in the Fermentation Brothe in vitro[J]. , 2016, 43(5): 1226-1231.

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The Study of Isolation, Identification of S.cerevisiae from Dairy Feed and its Antibacterical Activity in the Fermentation Brothe in vitro

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