猫传染性鼻气管炎病毒实时荧光定量PCR检测方法的建立及应用

doi:10.16431/j.cnki.1671-7236.2016.05.009

Abstract

Abstract: Feline herpesvirus type 1 (FHV-1) is the pathogeny of feline infectious rhinotracheitis.Based on the sequence of gD gene of FHV-1, one pair of primers(FHV-1F and FHV-1R) and FHV-1Probe were designed for the quantitative Real-time PCR detection method.The results showed that the method could detect FHV-1 DNA specifically and the sensitivity was 75 fg/μL.The reaction efficiency reached 107%, and the R² value was 0.9965.The coefficient of variance(CV) was below 2%.And the method was more sensitive than common PCR.It was specific, stable, and suitable for clinical diagnosis, detection and epidemiological investigation.Using this method, the Shanghai area pet clinic censorship suspected FHV-1 infected samples were detected, and the positive rate reached 27.78%, which indicated that the incidence of FHV-1 in Shanghai area was at a high level.

Key words: FIR; quantitative Real-time PCR; detection

CLC Number:

S852.65

LIU Jian, XU Feng, YANG Xian-chao, LI Xin, DENG Bo, YANG De-quan, JU Hou-bin, GE Jie, GONG Guo-hua, ZHOU Jin-ping. Establishment and Application of Quantitative Real-time PCR Detection Method for Feline Infectious Rhinotracheitis Virus[J]. , 2016, 43(5): 1182-1187.

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Establishment and Application of Quantitative Real-time PCR Detection Method for Feline Infectious Rhinotracheitis Virus

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