贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测

doi:10.16431/j.cnki.1671-7236.2016.04.037

Abstract

Abstract: To find out the drug resistance and the distribution and prevalence of ESBLs genotypes of E.coli strains on scale pig farms in Guizhou, the antimicrobial susceptibility of 164 E.coli strains were determined.And the genotype of ESBLs was identified and determined by CLSI standard method, DNA was amplified and the 4 genotypes of drug resistance plasmid of ESBLs were analyzed by PCR.The results showed that the drug resistance rates of 164 E.coli strains to ten kinds of antimicrobial agents were ceftiofur 93.29%, ampicillin 87.19%, tetracycline 86.59%, gentamicin 81.10%, streptomycin 53.66%, polymyxin 51.83%, ciprofloxacin 53.05%, kanamycin 47.56%, chlortetracycline 34.76% and florfenicol 21.95%, respectively, and most of them were multiple drug resistance strains, during which there were 137 strains ESBLs positive, detection rate was 83.54%, and the detection rates of 4 regions were different.The detection rates of TEM, CTX-M-1, SHV and OXA-1 genes of 137 ESBLs-producing E.coli strains were 90.51%, 70.07%, 51.82% and 43.07%, respectively.And the detection rates were various in 4 regions.The results showed that the drug resistance of E.coli strains from scale pig farms was serious in Guizhou, and the detection rate of ESBLs strains was very high, the drug resistance gene detection rate was extremely high, and most drug resistance strains were the composite genotype drug resistant.To prevent and cure colibacillosis in veterinary clinic effectively, we should reinforce monitoring and studying about the ESBLs-producing drug resistance bacteria.

Key words: swine E.coli; drug resistance; ESBLs; genotype

CLC Number:

S852.61

CAO Min, TAN Ai-juan, LV Shi-ming, CHANG Xin, DING Chang-qing, CHEN Ting, WANG Zhen-yan. Resistance Analysis and Genotype Detection of ESBLs-producing Swine E.coli Strains in Parts of Guizhou Province[J]. , 2016, 43(4): 1098-1104.

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Resistance Analysis and Genotype Detection of ESBLs-producing Swine E.coli Strains in Parts of Guizhou Province

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