山羊呼吸系统疾病病原分析

doi:10.16431/j.cnki.1671-7236.2016.04.033

Abstract

Abstract: To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.

Key words: goat's respiratory diseases; pathogen analysis; Serratia marcescens; E.coli

CLC Number:

S856.3

TAO Li, LAN Xian-li, LI Jun, MA Chun-xia, LAN Jin-hong, HUANG Ming-xue, LAN Mei-yi, BI Bing-fen, CHEN Ze-xiang, YANG Wei. Pathogen Analysis of Goat's Respiratory Diseases[J]. , 2016, 43(4): 1072-1078.

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Pathogen Analysis of Goat's Respiratory Diseases

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